BMRB Entry 27575
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27575
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for transthyetin in 5% DMSO PubMed: 31502419
Deposition date: 2018-08-13 Original release date: 2018-09-12
Authors: Yu, Tsyr-Yan; Yen, Yueh-Jung; Chang, Yu; Huang, Shing-Jong
Citation: Liu, Yo-Tsen; Yen, Yueh-Jung; Ricardo, Frans; Chang, Yu; Wu, Pei-Hao; Huang, Shing-Jong; Lin, Kon-Ping; Yu, Tsyr-Yan. "Biophysical characterization and modulation of Transthyretin Ala97Ser" Ann. Clin. Transl. Neurol. 6, 1961-1970 (2019).
Assembly members:
wtTTR, polymer, 127 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b
Entity Sequences (FASTA):
wtTTR: GPTGTGESKCPLMVKVLDAV
RGSPAINVAVHVFRKAADDT
WEPFASGKTSESGELHGLTT
EEEFVEGIYKVEIDTKSYWK
ALGISPFHEHAEVVFTANDS
GPRRYTIAALLSPYSYSTTA
VVTNPKE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 125 |
15N chemical shifts | 116 |
1H chemical shifts | 116 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | wtTTR | 1 |
Entities:
Entity 1, wtTTR 127 residues - Formula weight is not available
1 | GLY | PRO | THR | GLY | THR | GLY | GLU | SER | LYS | CYS | ||||
2 | PRO | LEU | MET | VAL | LYS | VAL | LEU | ASP | ALA | VAL | ||||
3 | ARG | GLY | SER | PRO | ALA | ILE | ASN | VAL | ALA | VAL | ||||
4 | HIS | VAL | PHE | ARG | LYS | ALA | ALA | ASP | ASP | THR | ||||
5 | TRP | GLU | PRO | PHE | ALA | SER | GLY | LYS | THR | SER | ||||
6 | GLU | SER | GLY | GLU | LEU | HIS | GLY | LEU | THR | THR | ||||
7 | GLU | GLU | GLU | PHE | VAL | GLU | GLY | ILE | TYR | LYS | ||||
8 | VAL | GLU | ILE | ASP | THR | LYS | SER | TYR | TRP | LYS | ||||
9 | ALA | LEU | GLY | ILE | SER | PRO | PHE | HIS | GLU | HIS | ||||
10 | ALA | GLU | VAL | VAL | PHE | THR | ALA | ASN | ASP | SER | ||||
11 | GLY | PRO | ARG | ARG | TYR | THR | ILE | ALA | ALA | LEU | ||||
12 | LEU | SER | PRO | TYR | SER | TYR | SER | THR | THR | ALA | ||||
13 | VAL | VAL | THR | ASN | PRO | LYS | GLU |
Samples:
sample_1: wtTTR, [U-13C; U-15N; U-2H], 0.75 mM; sodium phosphate 25 mM; sodium chloride 100 mM; EDTA 0.5 mM; TCEP 1 mM; DMSO 5%
sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts