BMRB Entry 27580
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27580
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Title: Backbone 1H, 15N and 13C Chemical shifts of N-terminal domain of antitoxin VapB46 from Mycobacterium tuberculosis PubMed: 30576065
Deposition date: 2018-08-18 Original release date: 2019-01-02
Authors: Roy, Madhurima; De, Soumya; Das, Amit
Citation: Roy, Madhurima; Kundu, Anirban; Bhunia, Anirban; Das Gupta, Sujoy; De, Soumya; Das, Amit Kumar. "Structural characterization of VapB46 antitoxin from Mycobacterium tuberculosis: insights into VapB46-DNA binding." FEBS J. 286, 1174-1190 (2019).
Assembly members:
VapB46, polymer, 66 residues, Formula weight is not available
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30
Entity Sequences (FASTA):
VapB46: MRGSHHHHHHGSMTPTACAT
VSTMTSVGVRALRQRASELL
RRVEAGETIEITDRGRPVAL
LSPLPQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 148 |
| 15N chemical shifts | 46 |
| 1H chemical shifts | 46 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VapB46 | 1 |
Entities:
Entity 1, VapB46 66 residues - Formula weight is not available
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLY | SER | MET | THR | PRO | THR | ALA | CYS | ALA | THR | ||||
| 3 | VAL | SER | THR | MET | THR | SER | VAL | GLY | VAL | ARG | ||||
| 4 | ALA | LEU | ARG | GLN | ARG | ALA | SER | GLU | LEU | LEU | ||||
| 5 | ARG | ARG | VAL | GLU | ALA | GLY | GLU | THR | ILE | GLU | ||||
| 6 | ILE | THR | ASP | ARG | GLY | ARG | PRO | VAL | ALA | LEU | ||||
| 7 | LEU | SER | PRO | LEU | PRO | GLN |
Samples:
sample_1: VapB46, [U-13C; U-15N], 0.3 mM; potassium phosphate 50 mM; sodium chloride 50 mM; DTT 2 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Zhengrong and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts