BMRB Entry 27603
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27603
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Title: Partial backbone assignments of Cse4 protein in 5M urea PubMed: 30381395
Deposition date: 2018-09-11 Original release date: 2019-01-09
Authors: Kumar, Ashutosh; Malik, Nikita
Citation: Malik, Nikita; Dantu, Sarath Chandra; Shukla, Shivangi; Kombrabail, Mamta; Ghosh, Santanu Kumar; Krishnamoorthy, Guruswamy; Kumar, Ashutosh. "Conformational flexibility of histone variant CENP-A" J. Biol. Chem. 293, 20273-20284 (2018).
Assembly members:
Cse4, polymer, 229 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKS387
Entity Sequences (FASTA):
Cse4: MSSKQQWVSSAIQSDSSGRS
LSNVNRLAGDQQSINDRALS
LLQRTRATKNLFPRREERRR
YESSKSDLDIETDYEDQAGN
LEIETENEEEAEMETEVPAP
VRTHSYALDRYVRQKRREKQ
RKQSLKRVEKKYTPSELALY
EIRKYQRSTDLLISKIPFAR
LVKEVTDEFTTKDQDLRWQS
MAIMALQEASEAYLVGLLEH
TNLLALHAKRITIMKKDMQL
ARRIRGQFI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 4 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 65 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cse4 | 1 |
Entities:
Entity 1, Cse4 229 residues - Formula weight is not available
| 1 | MET | SER | SER | LYS | GLN | GLN | TRP | VAL | SER | SER | ||||
| 2 | ALA | ILE | GLN | SER | ASP | SER | SER | GLY | ARG | SER | ||||
| 3 | LEU | SER | ASN | VAL | ASN | ARG | LEU | ALA | GLY | ASP | ||||
| 4 | GLN | GLN | SER | ILE | ASN | ASP | ARG | ALA | LEU | SER | ||||
| 5 | LEU | LEU | GLN | ARG | THR | ARG | ALA | THR | LYS | ASN | ||||
| 6 | LEU | PHE | PRO | ARG | ARG | GLU | GLU | ARG | ARG | ARG | ||||
| 7 | TYR | GLU | SER | SER | LYS | SER | ASP | LEU | ASP | ILE | ||||
| 8 | GLU | THR | ASP | TYR | GLU | ASP | GLN | ALA | GLY | ASN | ||||
| 9 | LEU | GLU | ILE | GLU | THR | GLU | ASN | GLU | GLU | GLU | ||||
| 10 | ALA | GLU | MET | GLU | THR | GLU | VAL | PRO | ALA | PRO | ||||
| 11 | VAL | ARG | THR | HIS | SER | TYR | ALA | LEU | ASP | ARG | ||||
| 12 | TYR | VAL | ARG | GLN | LYS | ARG | ARG | GLU | LYS | GLN | ||||
| 13 | ARG | LYS | GLN | SER | LEU | LYS | ARG | VAL | GLU | LYS | ||||
| 14 | LYS | TYR | THR | PRO | SER | GLU | LEU | ALA | LEU | TYR | ||||
| 15 | GLU | ILE | ARG | LYS | TYR | GLN | ARG | SER | THR | ASP | ||||
| 16 | LEU | LEU | ILE | SER | LYS | ILE | PRO | PHE | ALA | ARG | ||||
| 17 | LEU | VAL | LYS | GLU | VAL | THR | ASP | GLU | PHE | THR | ||||
| 18 | THR | LYS | ASP | GLN | ASP | LEU | ARG | TRP | GLN | SER | ||||
| 19 | MET | ALA | ILE | MET | ALA | LEU | GLN | GLU | ALA | SER | ||||
| 20 | GLU | ALA | TYR | LEU | VAL | GLY | LEU | LEU | GLU | HIS | ||||
| 21 | THR | ASN | LEU | LEU | ALA | LEU | HIS | ALA | LYS | ARG | ||||
| 22 | ILE | THR | ILE | MET | LYS | LYS | ASP | MET | GLN | LEU | ||||
| 23 | ALA | ARG | ARG | ILE | ARG | GLY | GLN | PHE | ILE |
Samples:
sample_1: Cse4, [U-99% 13C; U-99% 15N], 700 uM; urea 5 M
sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts