BMRB Entry 27616

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CBX8 chromodomain
Published: 2018-12-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27616

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3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CBX8 chromodomain PubMed: 30597065

Deposition date: 2018-09-19 Original release date: 2018-12-17

Authors: Weaver, Tyler; Musselman, Catherine

Citation: Connelly, Katelyn; Weaver, Tyler; Alpsoy, Aktan; Gu, Brian; Musselman, Catherine; Dykhuizen, Emily. "Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association." Nucleic Acids Res. 47, 2289-2305 (2019).

Assembly members:
CBX8_CD, polymer, 55 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGSTag

Entity Sequences (FASTA):
CBX8_CD: GERVFAAEALLKRRIRKGRM EYLVKWKGWSQKYSTWEPEE NILDARLLAAFEERE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	101
15N chemical shifts	49
1H chemical shifts	49

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CBX8 CD	1

Entities:

Entity 1, CBX8 CD 55 residues - Formula weight is not available

1

GLY

GLU

ARG

VAL

PHE

ALA

GLU

ALA

LEU

2

LEU

LYS

ARG

ILE

ARG

LYS

GLY

ARG

MET

3

GLU

TYR

LEU

VAL

LYS

TRP

LYS

GLY

TRP

SER

4

GLN

LYS

TYR

SER

THR

TRP

GLU

PRO

GLU

5

ASN

ILE

LEU

ASP

ALA

ARG

LEU

ALA

6

PHE

GLU

ARG

GLU

Samples:

sample_1: CBX8 CD, [U-13C; U-15N], 275 uM; sodium chloride 100 mM; sodium phosphate 40 mM

sample_conditions_1: ionic strength: 0.14 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts