BMRB Entry 27621

Name: Backbone 1H, 15N, 13C assignment of DfdT337-K426.
Published: 2019-03-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27621

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 15N, 13C assignment of DfdT337-K426. PubMed: 30802457

Deposition date: 2018-09-24 Original release date: 2019-03-28

Authors: Acharya, Bidisha; Maiti, Snigdha; De, Soumya

Citation: Maiti, Snigdha; Acharya, Bidisha; Boorla, Veda Sheersh; Manna, Bharat; Ghosh, Amit; De, Soumya. "Dynamic Studies on Intrinsically Disordered Regions of Two Paralogous Transcription Factors Reveal Rigid Segments with Important Biological Functions" J. Mol. Biol. 431, 1353-1369 (2019).

Assembly members:
DFD, polymer, 90 residues, Formula weight is not available

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):
DFD: TDGERIIYPWMKKIHVAGVA NGSYQPGMEPKRQRTAYTRH QILELEKEFHYNRYLTRRRR IEIAHTLVLSERQIKIWFQN RRMKWKKDNK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	310
15N chemical shifts	89
1H chemical shifts	94

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DFD monomer	1

Entities:

Entity 1, DFD monomer 90 residues - Formula weight is not available

1	THR	ASP	GLY	GLU	ARG	ILE	ILE	TYR	PRO	TRP
2	MET	LYS	LYS	ILE	HIS	VAL	ALA	GLY	VAL	ALA
3	ASN	GLY	SER	TYR	GLN	PRO	GLY	MET	GLU	PRO
4	LYS	ARG	GLN	ARG	THR	ALA	TYR	THR	ARG	HIS
5	GLN	ILE	LEU	GLU	LEU	GLU	LYS	GLU	PHE	HIS
6	TYR	ASN	ARG	TYR	LEU	THR	ARG	ARG	ARG	ARG
7	ILE	GLU	ILE	ALA	HIS	THR	LEU	VAL	LEU	SER
8	GLU	ARG	GLN	ILE	LYS	ILE	TRP	PHE	GLN	ASN
9	ARG	ARG	MET	LYS	TRP	LYS	LYS	ASP	ASN	LYS

Samples:

sample_1: DFD, [U-100% 13C; U-100% 15N], 0.3 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.131, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	THR	ASP	GLY	GLU	ARG	ILE	ILE	TYR	PRO	TRP
2	MET	LYS	LYS	ILE	HIS	VAL	ALA	GLY	VAL	ALA
3	ASN	GLY	SER	TYR	GLN	PRO	GLY	MET	GLU	PRO
4	LYS	ARG	GLN	ARG	THR	ALA	TYR	THR	ARG	HIS
5	GLN	ILE	LEU	GLU	LEU	GLU	LYS	GLU	PHE	HIS
6	TYR	ASN	ARG	TYR	LEU	THR	ARG	ARG	ARG	ARG
7	ILE	GLU	ILE	ALA	HIS	THR	LEU	VAL	LEU	SER
8	GLU	ARG	GLN	ILE	LYS	ILE	TRP	PHE	GLN	ASN
9	ARG	ARG	MET	LYS	TRP	LYS	LYS	ASP	ASN	LYS

1	THR	ASP	GLY	GLU	ARG	ILE	ILE	TYR	PRO	TRP
2	MET	LYS	LYS	ILE	HIS	VAL	ALA	GLY	VAL	ALA
3	ASN	GLY	SER	TYR	GLN	PRO	GLY	MET	GLU	PRO
4	LYS	ARG	GLN	ARG	THR	ALA	TYR	THR	ARG	HIS
5	GLN	ILE	LEU	GLU	LEU	GLU	LYS	GLU	PHE	HIS
6	TYR	ASN	ARG	TYR	LEU	THR	ARG	ARG	ARG	ARG
7	ILE	GLU	ILE	ALA	HIS	THR	LEU	VAL	LEU	SER
8	GLU	ARG	GLN	ILE	LYS	ILE	TRP	PHE	GLN	ASN
9	ARG	ARG	MET	LYS	TRP	LYS	LYS	ASP	ASN	LYS

1	THR	ASP	GLY	GLU	ARG	ILE	ILE	TYR	PRO	TRP
2	MET	LYS	LYS	ILE	HIS	VAL	ALA	GLY	VAL	ALA
3	ASN	GLY	SER	TYR	GLN	PRO	GLY	MET	GLU	PRO
4	LYS	ARG	GLN	ARG	THR	ALA	TYR	THR	ARG	HIS
5	GLN	ILE	LEU	GLU	LEU	GLU	LYS	GLU	PHE	HIS
6	TYR	ASN	ARG	TYR	LEU	THR	ARG	ARG	ARG	ARG
7	ILE	GLU	ILE	ALA	HIS	THR	LEU	VAL	LEU	SER
8	GLU	ARG	GLN	ILE	LYS	ILE	TRP	PHE	GLN	ASN
9	ARG	ARG	MET	LYS	TRP	LYS	LYS	ASP	ASN	LYS