BMRB Entry 27651
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27651
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Title: Conformational Changes in the Cytoplasmic Region of KIR3DL1 upon Interaction with SHP-2 PubMed: 30773397
Deposition date: 2018-10-15 Original release date: 2019-02-22
Authors: Cheng, Hong; Schwell, Vered; Curtis, Brett; Fazlieva, Ruzaliya; Roder, Heinrich; Campbell, Kerry
Citation: Cheng, Hong; Schwell, Vered; Curtis, Brett; Fazlieva, Ruzaliya; Roder, Heinrich; Campbell, Kerry. "Conformational Changes in the Cytoplasmic Region of KIR3DL1 upon Interaction with SHP-2" Structure 27, 639-650 (2019).
Assembly members:
KIR3DL1-cyto, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32b
Entity Sequences (FASTA):
KIR3DL1-cyto: GSGMKETAAAKFERQDMDSP
DLGTDDDDKAMEFHLWCSNK
KNAAVMDQEPAGNRTANSED
SDEQDPEEVTYAQLDHCVFT
QRKITRPSQRPKTPPTDTIL
YTELPNAKPRSKVVSCP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 392 |
| 15N chemical shifts | 99 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KIR3DL1-cyto | 1 |
Entities:
Entity 1, KIR3DL1-cyto 117 residues - Formula weight is not available
| 1 | GLY | SER | GLY | MET | LYS | GLU | THR | ALA | ALA | ALA | ||||
| 2 | LYS | PHE | GLU | ARG | GLN | ASP | MET | ASP | SER | PRO | ||||
| 3 | ASP | LEU | GLY | THR | ASP | ASP | ASP | ASP | LYS | ALA | ||||
| 4 | MET | GLU | PHE | HIS | LEU | TRP | CYS | SER | ASN | LYS | ||||
| 5 | LYS | ASN | ALA | ALA | VAL | MET | ASP | GLN | GLU | PRO | ||||
| 6 | ALA | GLY | ASN | ARG | THR | ALA | ASN | SER | GLU | ASP | ||||
| 7 | SER | ASP | GLU | GLN | ASP | PRO | GLU | GLU | VAL | THR | ||||
| 8 | TYR | ALA | GLN | LEU | ASP | HIS | CYS | VAL | PHE | THR | ||||
| 9 | GLN | ARG | LYS | ILE | THR | ARG | PRO | SER | GLN | ARG | ||||
| 10 | PRO | LYS | THR | PRO | PRO | THR | ASP | THR | ILE | LEU | ||||
| 11 | TYR | THR | GLU | LEU | PRO | ASN | ALA | LYS | PRO | ARG | ||||
| 12 | SER | LYS | VAL | VAL | SER | CYS | PRO |
Samples:
sample_1: KIR3DL1-cyto, [U-100% 13C; U-100% 15N], 1 mM; HEPES 20 mM; sodium chloride 20 mM; EDTA 3 mM; DTT 3 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CON | sample_1 | isotropic | sample_conditions_1 |
| CANCO | sample_1 | isotropic | sample_conditions_1 |
| CANCOi | sample_1 | isotropic | sample_conditions_1 |
| CBCACO | sample_1 | isotropic | sample_conditions_1 |
| CBCANCO | sample_1 | isotropic | sample_conditions_1 |
| CCCON | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.134, Goddard - chemical shift assignment
Felix v2007, Accelrys Software Inc. - processing
NMR spectrometers:
- Bruker Avance 600 MHz