BMRB Entry 27659

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27659

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Title:
Backbone assignment of human ribonuclease 6
Deposition date:
2018-10-22
Original release date:
2018-11-28
Authors:
Bernard, David; Gagne, Donald; Letourneau, Myriam; Doucet, Nicolas
Citation:

Citation: Narayanan, Chitra; Bernard, David; Letourneau, Myriam; Gagnon, Jacinthe; Gagne, Donald; Bafna, Khushboo; Calmettes, Charles; Couture, Jean-Francois; Agarwal, Pratul; Doucet, Nicolas. "Insights into Structural and Dynamical Changes Experienced by Human RNase 6 upon Ligand Binding"  Biochemistry 59, 755-765 (2020).
PubMed: 31909602

Assembly members:

Assembly members:
RNase6, polymer, 127 residues, 14653 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNase6: WPKRLTKAHWFEIQHIQPSP LQCNRAMSGINNYTQHCKHQ NTFLHDSFQNVAAVCDLLSI VCKNRRHNCHQSSKPVNMTD CRLTSGKYPQCRYSAAAQYK FFIVACDPPQKSDPPYKLVP VHLDSIL

Data typeCount
13C chemical shifts257
15N chemical shifts137
1H chemical shifts146

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNase 6, Major_conformer1
2RNase 6, Minor_conformer1

Entities:

Entity 1, RNase 6, Major_conformer 127 residues - 14653 Da.

1   TRPPROLYSARGLEUTHRLYSALAHISTRP
2   PHEGLUILEGLNHISILEGLNPROSERPRO
3   LEUGLNCYSASNARGALAMETSERGLYILE
4   ASNASNTYRTHRGLNHISCYSLYSHISGLN
5   ASNTHRPHELEUHISASPSERPHEGLNASN
6   VALALAALAVALCYSASPLEULEUSERILE
7   VALCYSLYSASNARGARGHISASNCYSHIS
8   GLNSERSERLYSPROVALASNMETTHRASP
9   CYSARGLEUTHRSERGLYLYSTYRPROGLN
10   CYSARGTYRSERALAALAALAGLNTYRLYS
11   PHEPHEILEVALALACYSASPPROPROGLN
12   LYSSERASPPROPROTYRLYSLEUVALPRO
13   VALHISLEUASPSERILELEU

Related Database Links:

UNP Q93091
PDB 4x09 5oab
AlphaFold Q93091

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks