BMRB Entry 27659
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27659
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Title: Backbone assignment of human ribonuclease 6 PubMed: 31909602
Deposition date: 2018-10-22 Original release date: 2018-11-28
Authors: Bernard, David; Gagne, Donald; Letourneau, Myriam; Doucet, Nicolas
Citation: Narayanan, Chitra; Bernard, David; Letourneau, Myriam; Gagnon, Jacinthe; Gagne, Donald; Bafna, Khushboo; Calmettes, Charles; Couture, Jean-Francois; Agarwal, Pratul; Doucet, Nicolas. "Insights into Structural and Dynamical Changes Experienced by Human RNase 6 upon Ligand Binding" Biochemistry 59, 755-765 (2020).
Assembly members:
RNase6, polymer, 127 residues, 14653 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJ414
Entity Sequences (FASTA):
RNase6: WPKRLTKAHWFEIQHIQPSP
LQCNRAMSGINNYTQHCKHQ
NTFLHDSFQNVAAVCDLLSI
VCKNRRHNCHQSSKPVNMTD
CRLTSGKYPQCRYSAAAQYK
FFIVACDPPQKSDPPYKLVP
VHLDSIL
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 257 |
15N chemical shifts | 137 |
1H chemical shifts | 146 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RNase 6, Major_conformer | 1 |
2 | RNase 6, Minor_conformer | 1 |
Entities:
Entity 1, RNase 6, Major_conformer 127 residues - 14653 Da.
1 | TRP | PRO | LYS | ARG | LEU | THR | LYS | ALA | HIS | TRP | ||||
2 | PHE | GLU | ILE | GLN | HIS | ILE | GLN | PRO | SER | PRO | ||||
3 | LEU | GLN | CYS | ASN | ARG | ALA | MET | SER | GLY | ILE | ||||
4 | ASN | ASN | TYR | THR | GLN | HIS | CYS | LYS | HIS | GLN | ||||
5 | ASN | THR | PHE | LEU | HIS | ASP | SER | PHE | GLN | ASN | ||||
6 | VAL | ALA | ALA | VAL | CYS | ASP | LEU | LEU | SER | ILE | ||||
7 | VAL | CYS | LYS | ASN | ARG | ARG | HIS | ASN | CYS | HIS | ||||
8 | GLN | SER | SER | LYS | PRO | VAL | ASN | MET | THR | ASP | ||||
9 | CYS | ARG | LEU | THR | SER | GLY | LYS | TYR | PRO | GLN | ||||
10 | CYS | ARG | TYR | SER | ALA | ALA | ALA | GLN | TYR | LYS | ||||
11 | PHE | PHE | ILE | VAL | ALA | CYS | ASP | PRO | PRO | GLN | ||||
12 | LYS | SER | ASP | PRO | PRO | TYR | LYS | LEU | VAL | PRO | ||||
13 | VAL | HIS | LEU | ASP | SER | ILE | LEU |
Samples:
sample_1: RNase6, [U-99% 13C; U-99% 15N], 680 uM; D2O, [U-99% 2H], 10%; sodium acetate 15 mM
sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4, CCPN - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts