BMRB Entry 27663

Title:
HKU4 NSP3 C Domain
Deposition date:
2018-10-26
Original release date:
2021-07-21
Authors:
Staup, Andrew; De Silva, Ivon; Catt, Justin; Tan, Xuan; Hammond, Robert; Ahmadvand, Parvaneh; Cowan, Jaden; Crisp, Eric; Cuny, Zachary; Konutham, Amareshwari; Mushimiyimana, Anne; Parker, Candace; Prasannakumar, Geethika; Rojas, Edwin; Subedi, Ashok; Zaharias, Steve; Jablonsky, Michael; Shin, Ronald; Johnson, Margaret
Citation:

Citation: Staup, Andrew; De Silva, Ivon; Catt, Justin; Tan, Xuan; Hammond, Robert; Johnson, Margaret. "Structure of the SARS-Unique Domain C From the Bat Coronavirus HKU4"  Nat. Prod. Commun. 14, .-. (2019).
PubMed: 32395093

Assembly members:

Assembly members:
HKU4C, polymer, 81 residues, 9111.31 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 694007   Superkingdom: virus   Kingdom: ssRNA virus   Genus/species: betacoronavirus Tylonycteris bat coronavirus HKU4

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b-TEV

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts324
15N chemical shifts85
1H chemical shifts535

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HKU4C1

Entities:

Entity 1, HKU4C 81 residues - 9111.31 Da.

First three residues (-3 to -1) are remnants of a non-native affinity tag. Residues Q 1445 through A 1522 are native to non-structural protein 3 of HKU4 and are numbered based on the beginning of nsp3.

1   SERHISMETGLNTHRPROGLUTHRALAPHE
2   ILEASNASNVALTHRSERASNGLYGLYTYR
3   HISSERTRPHISLEUVALSERGLYASPLEU
4   ILEVALLYSASPVALCYSTYRLYSLYSLEU
5   LEUHISTRPSERGLYGLNTHRILECYSTYR
6   ALAASPASNLYSPHETYRVALVALLYSASN
7   ASPVALALALEUPROPHESERASPLEUGLU
8   ALACYSARGALATYRLEUTHRSERARGALA
9   ALA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks