BMRB Entry 27669
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27669
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Title: Amide chemical shifts of full-length human HuR PubMed: 30418581
Deposition date: 2018-10-30 Original release date: 2018-11-14
Authors: Sattler, Michael; Schlundt, Andreas; Pabis, Marta; Popowicz, Grzegorz
Citation: Pabis, Marta; Popowicz, Grzegorz; Stehle, Ralf; Fernandez-Ramos, David; Asami, Sam; Warner, Lisa; Garcia-Maurino, Sofia; Schlundt, Andreas; Martinez-Chantar, Maria; Diaz-Moreno, Irene; Sattler, Michael. "HuR biological function involves RRM3-mediated dimerization and RNA binding by all three RRMs." Nucleic Acids Res. 47, 1011-1029 (2019).
Assembly members:
HuR_FL, polymer, 329 residues, 36317.13 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM44
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 212 |
| 1H chemical shifts | 212 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HuR FL | 1 |
Entities:
Entity 1, HuR FL 329 residues - 36317.13 Da.
GPMA is from cloning
| 1 | GLY | PRO | MET | ALA | SER | ASN | GLY | TYR | GLU | ASP | ||||
| 2 | HIS | MET | ALA | GLU | ASP | CYS | ARG | GLY | ASP | ILE | ||||
| 3 | GLY | ARG | THR | ASN | LEU | ILE | VAL | ASN | TYR | LEU | ||||
| 4 | PRO | GLN | ASN | MET | THR | GLN | ASP | GLU | LEU | ARG | ||||
| 5 | SER | LEU | PHE | SER | SER | ILE | GLY | GLU | VAL | GLU | ||||
| 6 | SER | ALA | LYS | LEU | ILE | ARG | ASP | LYS | VAL | ALA | ||||
| 7 | GLY | HIS | SER | LEU | GLY | TYR | GLY | PHE | VAL | ASN | ||||
| 8 | TYR | VAL | THR | ALA | LYS | ASP | ALA | GLU | ARG | ALA | ||||
| 9 | ILE | ASN | THR | LEU | ASN | GLY | LEU | ARG | LEU | GLN | ||||
| 10 | SER | LYS | THR | ILE | LYS | VAL | SER | TYR | ALA | ARG | ||||
| 11 | PRO | SER | SER | GLU | VAL | ILE | LYS | ASP | ALA | ASN | ||||
| 12 | LEU | TYR | ILE | SER | GLY | LEU | PRO | ARG | THR | MET | ||||
| 13 | THR | GLN | LYS | ASP | VAL | GLU | ASP | MET | PHE | SER | ||||
| 14 | ARG | PHE | GLY | ARG | ILE | ILE | ASN | SER | ARG | VAL | ||||
| 15 | LEU | VAL | ASP | GLN | THR | THR | GLY | LEU | SER | ARG | ||||
| 16 | GLY | VAL | ALA | PHE | ILE | ARG | PHE | ASP | LYS | ARG | ||||
| 17 | SER | GLU | ALA | GLU | GLU | ALA | ILE | THR | SER | PHE | ||||
| 18 | ASN | GLY | HIS | LYS | PRO | PRO | GLY | SER | SER | GLU | ||||
| 19 | PRO | ILE | THR | VAL | LYS | PHE | ALA | ALA | ASN | PRO | ||||
| 20 | ASN | GLN | ASN | LYS | ASN | VAL | ALA | LEU | LEU | SER | ||||
| 21 | GLN | LEU | TYR | HIS | SER | PRO | ALA | ARG | ARG | PHE | ||||
| 22 | GLY | GLY | PRO | VAL | HIS | HIS | GLN | ALA | GLN | ARG | ||||
| 23 | PHE | ARG | PHE | SER | PRO | MET | GLY | VAL | ASP | HIS | ||||
| 24 | MET | SER | GLY | LEU | SER | GLY | VAL | ASN | VAL | PRO | ||||
| 25 | GLY | ASN | ALA | SER | SER | GLY | TRP | CYS | ILE | PHE | ||||
| 26 | ILE | TYR | ASN | LEU | GLY | GLN | ASP | ALA | ASP | GLU | ||||
| 27 | GLY | ILE | LEU | TRP | GLN | MET | PHE | GLY | PRO | PHE | ||||
| 28 | GLY | ALA | VAL | THR | ASN | VAL | LYS | VAL | ILE | ARG | ||||
| 29 | ASP | PHE | ASN | THR | ASN | LYS | CYS | LYS | GLY | PHE | ||||
| 30 | GLY | PHE | VAL | THR | MET | THR | ASN | TYR | GLU | GLU | ||||
| 31 | ALA | ALA | MET | ALA | ILE | ALA | SER | LEU | ASN | GLY | ||||
| 32 | TYR | ARG | LEU | GLY | ASP | LYS | ILE | LEU | GLN | VAL | ||||
| 33 | SER | PHE | LYS | THR | ASN | LYS | SER | HIS | LYS |
Samples:
sample_1: HuR FL, [U-15N; U-2H], 20 uM; sodium phosphate 20 mM; sodium chloride 200 mM; DTT, [U-2H], 5 mM; EDTA 1 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
CCPNMR_Analysis v2.4, CCPN - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts