BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27693

Title: Backbone and Sidechian 1H, 13C, and 15N Chemical Shift Assignments for Escherichia Coli Periplasmic Chaperone HdeA at pH 1.5   PubMed: 30573682

Deposition date: 2018-11-19 Original release date: 2018-12-19

Authors: Yu, Xing-Chi; Jin, Changwen; Hu, Yunfei

Citation: Yu, Xing-Chi; Hu, Yunfei; Ding, Jienv; Li, Hongwei; Jin, Changwen. "Structural basis and mechanism of the unfolding-induced activation of HdeA, a bacterial acid response chaperone"  J. Biol. Chem. 294, 3192-3206 (2019).

Assembly members:
HdeA_F28W, polymer, 89 residues, 9779.95 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a(+)

Entity Sequences (FASTA):
HdeA_F28W: ADAQKAADNKKPVNSWTCED FLAVDESWQPTAVGFAEALN NKDKPEDAVLDVQGIATVTP AIVQACTQDKQANFKDKVKG EWDKIKKDM

Data sets:
Data typeCount
13C chemical shifts344
15N chemical shifts84
1H chemical shifts531

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HdeA monomer1

Entities:

Entity 1, HdeA monomer 89 residues - 9779.95 Da.

The sequence is numbered according to the mature protein in which the signal peptide is cleaved.

1   ALAASPALAGLNLYSALAALAASPASNLYS
2   LYSPROVALASNSERTRPTHRCYSGLUASP
3   PHELEUALAVALASPGLUSERTRPGLNPRO
4   THRALAVALGLYPHEALAGLUALALEUASN
5   ASNLYSASPLYSPROGLUASPALAVALLEU
6   ASPVALGLNGLYILEALATHRVALTHRPRO
7   ALAILEVALGLNALACYSTHRGLNASPLYS
8   GLNALAASNPHELYSASPLYSVALLYSGLY
9   GLUTRPASPLYSILELYSLYSASPMET

Samples:

sample_1: HdeA_F28W, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 90%; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM; citric acid 45 mM; DSS 0.01 % w/v

sample_conditions_1: ionic strength: 0.15 M; pH: 1.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMRView vv5.0.4, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts