BMRB Entry 27697

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27697

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Title:
1H, 15N, 13C backbone resonance assignments of the D10N variant of beta-phosphoglucomutase in a transition state analogue complex with glucose 6-phosphate and AlF4-
Deposition date:
2018-11-20
Original release date:
2021-11-29
Authors:
Robertson, Angus; Baxter, Nicola
Citation:

Citation: Robertson, Angus; Wilson, Alex; Burn, Matthew; Cliff, Matthew; Popelier, Paul; Waltho, Jonathan. "The Relationship between Enzyme Conformational Change, Proton Transfer, and Phosphoryl Transfer in beta-Phosphoglucomutase"  ACS Catal. 11, 12840-12849 (2021).

Assembly members:

Assembly members:
beta-phosphoglucomutase, polymer, 221 residues, 24209.65 Da.
entity_G6P, non-polymer, 260.136 Da.
entity_MG, non-polymer, 24.305 Da.
entity_ALF, non-polymer, 102.975 Da.

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta-phosphoglucomutase: MFKAVLFDLNGVITDTAEYH FRAWKALAEEIGINGVDRQF NEQLKGVSREDSLQKILDLA DKKVSAEEFKELAKRKNDNY VKMIQDVSPADVYPGILQLL KDLRSNKIKIALASASKNGP FLLERMNLTGYFDAIADPAE VAASKPAPDIFIAAAHAVGV APSESIGLEDSQAGIQAIKD SGALPIGVGRPEDLGDDIVI VPDTSHYTLEFLKEVWLQKQ K

Data sets:
Data typeCount
13C chemical shifts644
15N chemical shifts210
19F chemical shifts4
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1bPGM(D10N)1
2G6P2
3Mg3
4ALF4

Entities:

Entity 1, bPGM(D10N) 221 residues - 24209.65 Da.

1   METPHELYSALAVALLEUPHEASPLEUASN
2   GLYVALILETHRASPTHRALAGLUTYRHIS
3   PHEARGALATRPLYSALALEUALAGLUGLU
4   ILEGLYILEASNGLYVALASPARGGLNPHE
5   ASNGLUGLNLEULYSGLYVALSERARGGLU
6   ASPSERLEUGLNLYSILELEUASPLEUALA
7   ASPLYSLYSVALSERALAGLUGLUPHELYS
8   GLULEUALALYSARGLYSASNASPASNTYR
9   VALLYSMETILEGLNASPVALSERPROALA
10   ASPVALTYRPROGLYILELEUGLNLEULEU
11   LYSASPLEUARGSERASNLYSILELYSILE
12   ALALEUALASERALASERLYSASNGLYPRO
13   PHELEULEUGLUARGMETASNLEUTHRGLY
14   TYRPHEASPALAILEALAASPPROALAGLU
15   VALALAALASERLYSPROALAPROASPILE
16   PHEILEALAALAALAHISALAVALGLYVAL
17   ALAPROSERGLUSERILEGLYLEUGLUASP
18   SERGLNALAGLYILEGLNALAILELYSASP
19   SERGLYALALEUPROILEGLYVALGLYARG
20   PROGLUASPLEUGLYASPASPILEVALILE
21   VALPROASPTHRSERHISTYRTHRLEUGLU
22   PHELEULYSGLUVALTRPLEUGLNLYSGLN
23   LYS

Entity 2, G6P - C6 H13 O9 P - 260.136 Da.

1   G6P

Entity 3, Mg - Mg - 24.305 Da.

1   MG

Entity 4, ALF - Al F4 - 102.975 Da.

1   ALF

Related Database Links:

BMRB 27174
UNP P71447
AlphaFold P71447

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks