BMRB Entry 27706
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27706
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Title: EZH2 SANT1 PubMed: 30700785
Deposition date: 2018-11-28 Original release date: 2018-12-19
Authors: Weaver, Tyler; Musselman, Catherine
Citation: Weaver, Tyler; Liu, Jiachen; Connelly, Katelyn; Coble, Chris; Varzavand, Katayoun; Dykhuizen, Emily; Musselman, Catherine. "The EZH2 SANT1 domains is a histone reader domain providing sensitivity to the modification state of the H4 tail" Sci. Rep. 9, 987-987 (2019).
Assembly members:
EZH2_SANT1, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST15
Entity Sequences (FASTA):
EZH2_SANT1: GPQDGTFIEELIKNYDGKVH
GDRECGFINDEIFVELVNAL
GQYNDDDDDDDGDDPEEREE
KQKDLEDHRDDKESRPPRKF
PSDKIFEAISSMFPDKGTAE
ELKEKYKELTEQQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 252 |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 84 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EZH2 SANT1 | 1 |
Entities:
Entity 1, EZH2 SANT1 113 residues - Formula weight is not available
| 1 | GLY | PRO | GLN | ASP | GLY | THR | PHE | ILE | GLU | GLU | ||||
| 2 | LEU | ILE | LYS | ASN | TYR | ASP | GLY | LYS | VAL | HIS | ||||
| 3 | GLY | ASP | ARG | GLU | CYS | GLY | PHE | ILE | ASN | ASP | ||||
| 4 | GLU | ILE | PHE | VAL | GLU | LEU | VAL | ASN | ALA | LEU | ||||
| 5 | GLY | GLN | TYR | ASN | ASP | ASP | ASP | ASP | ASP | ASP | ||||
| 6 | ASP | GLY | ASP | ASP | PRO | GLU | GLU | ARG | GLU | GLU | ||||
| 7 | LYS | GLN | LYS | ASP | LEU | GLU | ASP | HIS | ARG | ASP | ||||
| 8 | ASP | LYS | GLU | SER | ARG | PRO | PRO | ARG | LYS | PHE | ||||
| 9 | PRO | SER | ASP | LYS | ILE | PHE | GLU | ALA | ILE | SER | ||||
| 10 | SER | MET | PHE | PRO | ASP | LYS | GLY | THR | ALA | GLU | ||||
| 11 | GLU | LEU | LYS | GLU | LYS | TYR | LYS | GLU | LEU | THR | ||||
| 12 | GLU | GLN | GLN |
Samples:
sample_1: EZH2 SANT1, [U-100% 13C; U-100% 15N], 700 mM; NaCL 150 mM
sample_2: EZH2 SANT1, [U-100% 13C; U-100% 15N], 715 uM; NaCL 150 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CCPNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts