BMRB Entry 27717
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27717
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Androgen Receptor (AR) polyQ-derived peptide L4Q20 PubMed: 31048691
Deposition date: 2018-12-05 Original release date: 2019-06-13
Authors: Salvatella, Xavier; Escobedo, Albert
Citation: Escobedo, Albert; Topal, Busra; Kunze, Micha; Aranda, Juan; Chiesa, Giulio; Mungianu, Daniele; Bernardo-Seisdedos, Ganeko; Eftekharzadeh, Bahareh; Gairi, Margarida; Pierattelli, Roberta; Felli, Isabella; Diercks, Tammo; Millet, Oscar; Garcia, Jesus; Orozco, Modesto; Crehuet, Ramon; Lindorff-Larsen, Kresten; Salvatella, Xavier. "Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor." Nat. Commun. 10, 2034-2034 (2019).
Assembly members:
L4Q20, polymer, 32 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST17
Entity Sequences (FASTA):
L4Q20: KKPGASLLLLQQQQQQQQQQ
QQQQQQQQQQKK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 75 |
15N chemical shifts | 30 |
1H chemical shifts | 30 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | L4Q20 | 1 |
Entities:
Entity 1, L4Q20 32 residues - Formula weight is not available
1 | LYS | LYS | PRO | GLY | ALA | SER | LEU | LEU | LEU | LEU | ||||
2 | GLN | GLN | GLN | GLN | GLN | GLN | GLN | GLN | GLN | GLN | ||||
3 | GLN | GLN | GLN | GLN | GLN | GLN | GLN | GLN | GLN | GLN | ||||
4 | LYS | LYS |
Samples:
sample_1: L4Q20, [U-95% 13C; U-95% 15N], 100 uM; DSS 10 uM; sodium phosphate 20 mM
sample_conditions_1: ionic strength: 0.09 M; pH: 7.4; pressure: 1 atm; temperature: 278 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HCACO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CcpNmr_Analysis, CCPN - chemical shift assignment
TopSpin, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts