BMRB Entry 27743

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27743

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
hSmad2-beta MH1 domain
Deposition date:
2018-12-24
Original release date:
2019-09-23
Authors:
Macias, Maria; Martin, Pau; Aragon, Eric; Ruiz, Lidia
Citation:

Citation: Aragon, Eric; Wang, Qiong; Zou, Yilong; Morgani, Sophie; Ruiz, Lidia; Kaczmarska, Zuzanna; Su, Jie; Torner, Carles; Tian, Lin; Hu, Jing; Shu, Weiping; Agrawal, Saloni; Gomes, Tiago; Marquez, Jose; Hadjantonakis, Anna-Katerina; Macias, Maria; Massague, Joan. "Structural basis for distinct roles of SMAD2 and SMAD3 in FOXH1 pioneer-directed TGF-beta signaling"  Genes Dev. 33, 1506-1524 (2019).
PubMed: 31582430

Assembly members:

Assembly members:
smad2, polymer, 149 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
smad2: MSSILPFTPPVVKRLLGWKK SAGGSGGAGGGEQNGQEEKW CEKAVKSLVKKLKKTGRLDE LEKAITTQNCNTKCVTIPRS LDGRLQVSHRKGLPHVIYCR LWRWPDLHSHHELKAIENCE YAFNLKKDEVCVNPYHYQRV ETPVLPPSS

Data sets:
Data typeCount
13C chemical shifts250
15N chemical shifts120
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1smad21

Entities:

Entity 1, smad2 149 residues - Formula weight is not available

This is an isoform lacking a 30aa exon. Sequence numbering is done as in the reference isoform. This is why the assignment skips residues 79 to 108.

1   METSERSERILELEUPROPHETHRPROPRO
2   VALVALLYSARGLEULEUGLYTRPLYSLYS
3   SERALAGLYGLYSERGLYGLYALAGLYGLY
4   GLYGLUGLNASNGLYGLNGLUGLULYSTRP
5   CYSGLULYSALAVALLYSSERLEUVALLYS
6   LYSLEULYSLYSTHRGLYARGLEUASPGLU
7   LEUGLULYSALAILETHRTHRGLNASNCYS
8   ASNTHRLYSCYSVALTHRILEPROARGSER
9   LEUASPGLYARGLEUGLNVALSERHISARG
10   LYSGLYLEUPROHISVALILETYRCYSARG
11   LEUTRPARGTRPPROASPLEUHISSERHIS
12   HISGLULEULYSALAILEGLUASNCYSGLU
13   TYRALAPHEASNLEULYSLYSASPGLUVAL
14   CYSVALASNPROTYRHISTYRGLNARGVAL
15   GLUTHRPROVALLEUPROPROSERSER

Related Database Links:

UNP Q15796-2
AlphaFold Q15796

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks