BMRB Entry 27744

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR27744

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Title:
Backbone chemical shift assignments of translation initiation factor 3 from Pseudomonas aeruginosa
Deposition date:
2018-12-26
Original release date:
2020-02-28
Authors:
Zhang, Yonghong
Citation:

Citation: Li, Libo; Palmer, Stephanie; Gomez, Elizabeth; Mendiola, Frank; Wang, Tianzhi; Bullard, James; Zhang, Yonghong. "Backbone chemical shift assignments of translation initiation factor 3 from Pseudomonas aeruginosa"  Biomol. NMR Assignments 14, 93-97 (2020).
PubMed: 31902070

Assembly members:

Assembly members:
PaIF3, polymer, 193 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PaIF3: MASIIKREMRQDKRAQPKPP INENISAREVRLIGADGQQV GVVSIDEAIRLAEEAKLDLV EISADAVPPVCRIMDYGKHL FEKKKQAAVAKKNQKQAQVK EIKFRPGTEEGDYQVKLRNL VRFLSEGDKAKVSLRFRGRE MAHQELGMELLKRVEADLVE YGTVEQHPKLEGRQLMMVIA PKKKKLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts677
15N chemical shifts171
1H chemical shifts1145

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PaIF31

Entities:

Entity 1, PaIF3 193 residues - Formula weight is not available

The first three residues (MAS) are from cloning artifacts. The last 8 residues (LEHHHHHH) represent a non-native affinity tag. This is a bacterial ribosome binding protein from Pseudomonas aeruginosa.

1   METALASERILEILELYSARGGLUMETARG
2   GLNASPLYSARGALAGLNPROLYSPROPRO
3   ILEASNGLUASNILESERALAARGGLUVAL
4   ARGLEUILEGLYALAASPGLYGLNGLNVAL
5   GLYVALVALSERILEASPGLUALAILEARG
6   LEUALAGLUGLUALALYSLEUASPLEUVAL
7   GLUILESERALAASPALAVALPROPROVAL
8   CYSARGILEMETASPTYRGLYLYSHISLEU
9   PHEGLULYSLYSLYSGLNALAALAVALALA
10   LYSLYSASNGLNLYSGLNALAGLNVALLYS
11   GLUILELYSPHEARGPROGLYTHRGLUGLU
12   GLYASPTYRGLNVALLYSLEUARGASNLEU
13   VALARGPHELEUSERGLUGLYASPLYSALA
14   LYSVALSERLEUARGPHEARGGLYARGGLU
15   METALAHISGLNGLULEUGLYMETGLULEU
16   LEULYSARGVALGLUALAASPLEUVALGLU
17   TYRGLYTHRVALGLUGLNHISPROLYSLEU
18   GLUGLYARGGLNLEUMETMETVALILEALA
19   PROLYSLYSLYSLYSLEUGLUHISHISHIS
20   HISHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks