BMRB Entry 27754

Name: The cytoplasm-entry domain of antibacterial CdiA is a dynamic alpha-helical bundle with disulfide-dependent structural features
Published: 2019-01-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27754

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The cytoplasm-entry domain of antibacterial CdiA is a dynamic alpha-helical bundle with disulfide-dependent structural features

Deposition date:

2019-01-16

Original release date:

2019-01-18

Authors:

Bartelli, Nicholas; Sun, Sheng; Gucinski, Grant; Zhou, Hongjun; Song, Kiho; Hayes, Christopher; Dahlquist, Frederick

Citation:

Citation: Bartelli, Nicholas; Sun, Sheng; Gucinski, Grant; Zhou, Hongjun; Song, Kiho; Hayes, Christopher; Dahlquist, Frederick. "The Cytoplasm-Entry Domain of Antibacterial CdiA Is a Dynamic alpha-Helical Bundle with Disulfide-Dependent Structural Features" J. Mol. Biol. 431, .-3203 (3216).
PubMed: 31181288

Assembly members:

Assembly members:
CdiA-CT_C21S_C27S, polymer, 150 residues, 15911 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CdiA-CT_C21S_C27S: VENNFLTADQIDSFAAKAKG SEVRGDSKQIVKEMEELSLK QQQEMIAVCATNPAACKEKF GDIPAKGMLVREAIDRVLGT DVPSAMKNDMSSLLAQQIEA EGVVTSTEFASQLQNRYGID KQQAEILAVAALGAVTGGMG KSGTSTVTKN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	424
15N chemical shifts	138
1H chemical shifts	822

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CdiA-CT C21S C27S	1

Entities:

Entity 1, CdiA-CT C21S C27S 150 residues - 15911 Da.

1	VAL	GLU	ASN	ASN	PHE	LEU	THR	ALA	ASP	GLN
2	ILE	ASP	SER	PHE	ALA	ALA	LYS	ALA	LYS	GLY
3	SER	GLU	VAL	ARG	GLY	ASP	SER	LYS	GLN	ILE
4	VAL	LYS	GLU	MET	GLU	GLU	LEU	SER	LEU	LYS
5	GLN	GLN	GLN	GLU	MET	ILE	ALA	VAL	CYS	ALA
6	THR	ASN	PRO	ALA	ALA	CYS	LYS	GLU	LYS	PHE
7	GLY	ASP	ILE	PRO	ALA	LYS	GLY	MET	LEU	VAL
8	ARG	GLU	ALA	ILE	ASP	ARG	VAL	LEU	GLY	THR
9	ASP	VAL	PRO	SER	ALA	MET	LYS	ASN	ASP	MET
10	SER	SER	LEU	LEU	ALA	GLN	GLN	ILE	GLU	ALA
11	GLU	GLY	VAL	VAL	THR	SER	THR	GLU	PHE	ALA
12	SER	GLN	LEU	GLN	ASN	ARG	TYR	GLY	ILE	ASP
13	LYS	GLN	GLN	ALA	GLU	ILE	LEU	ALA	VAL	ALA
14	ALA	LEU	GLY	ALA	VAL	THR	GLY	GLY	MET	GLY
15	LYS	SER	GLY	THR	SER	THR	VAL	THR	LYS	ASN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

BMRB Entry 27754

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	VAL	GLU	ASN	ASN	PHE	LEU	THR	ALA	ASP	GLN
2	ILE	ASP	SER	PHE	ALA	ALA	LYS	ALA	LYS	GLY
3	SER	GLU	VAL	ARG	GLY	ASP	SER	LYS	GLN	ILE
4	VAL	LYS	GLU	MET	GLU	GLU	LEU	SER	LEU	LYS
5	GLN	GLN	GLN	GLU	MET	ILE	ALA	VAL	CYS	ALA
6	THR	ASN	PRO	ALA	ALA	CYS	LYS	GLU	LYS	PHE
7	GLY	ASP	ILE	PRO	ALA	LYS	GLY	MET	LEU	VAL
8	ARG	GLU	ALA	ILE	ASP	ARG	VAL	LEU	GLY	THR
9	ASP	VAL	PRO	SER	ALA	MET	LYS	ASN	ASP	MET
10	SER	SER	LEU	LEU	ALA	GLN	GLN	ILE	GLU	ALA
11	GLU	GLY	VAL	VAL	THR	SER	THR	GLU	PHE	ALA
12	SER	GLN	LEU	GLN	ASN	ARG	TYR	GLY	ILE	ASP
13	LYS	GLN	GLN	ALA	GLU	ILE	LEU	ALA	VAL	ALA
14	ALA	LEU	GLY	ALA	VAL	THR	GLY	GLY	MET	GLY
15	LYS	SER	GLY	THR	SER	THR	VAL	THR	LYS	ASN

1	VAL	GLU	ASN	ASN	PHE	LEU	THR	ALA	ASP	GLN
2	ILE	ASP	SER	PHE	ALA	ALA	LYS	ALA	LYS	GLY
3	SER	GLU	VAL	ARG	GLY	ASP	SER	LYS	GLN	ILE
4	VAL	LYS	GLU	MET	GLU	GLU	LEU	SER	LEU	LYS
5	GLN	GLN	GLN	GLU	MET	ILE	ALA	VAL	CYS	ALA
6	THR	ASN	PRO	ALA	ALA	CYS	LYS	GLU	LYS	PHE
7	GLY	ASP	ILE	PRO	ALA	LYS	GLY	MET	LEU	VAL
8	ARG	GLU	ALA	ILE	ASP	ARG	VAL	LEU	GLY	THR
9	ASP	VAL	PRO	SER	ALA	MET	LYS	ASN	ASP	MET
10	SER	SER	LEU	LEU	ALA	GLN	GLN	ILE	GLU	ALA
11	GLU	GLY	VAL	VAL	THR	SER	THR	GLU	PHE	ALA
12	SER	GLN	LEU	GLN	ASN	ARG	TYR	GLY	ILE	ASP
13	LYS	GLN	GLN	ALA	GLU	ILE	LEU	ALA	VAL	ALA
14	ALA	LEU	GLY	ALA	VAL	THR	GLY	GLY	MET	GLY
15	LYS	SER	GLY	THR	SER	THR	VAL	THR	LYS	ASN

1	VAL	GLU	ASN	ASN	PHE	LEU	THR	ALA	ASP	GLN
2	ILE	ASP	SER	PHE	ALA	ALA	LYS	ALA	LYS	GLY
3	SER	GLU	VAL	ARG	GLY	ASP	SER	LYS	GLN	ILE
4	VAL	LYS	GLU	MET	GLU	GLU	LEU	SER	LEU	LYS
5	GLN	GLN	GLN	GLU	MET	ILE	ALA	VAL	CYS	ALA
6	THR	ASN	PRO	ALA	ALA	CYS	LYS	GLU	LYS	PHE
7	GLY	ASP	ILE	PRO	ALA	LYS	GLY	MET	LEU	VAL
8	ARG	GLU	ALA	ILE	ASP	ARG	VAL	LEU	GLY	THR
9	ASP	VAL	PRO	SER	ALA	MET	LYS	ASN	ASP	MET
10	SER	SER	LEU	LEU	ALA	GLN	GLN	ILE	GLU	ALA
11	GLU	GLY	VAL	VAL	THR	SER	THR	GLU	PHE	ALA
12	SER	GLN	LEU	GLN	ASN	ARG	TYR	GLY	ILE	ASP
13	LYS	GLN	GLN	ALA	GLU	ILE	LEU	ALA	VAL	ALA
14	ALA	LEU	GLY	ALA	VAL	THR	GLY	GLY	MET	GLY
15	LYS	SER	GLY	THR	SER	THR	VAL	THR	LYS	ASN