BMRB Entry 27759
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27759
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Title: 1H and 15N Chemical assignment for NSL2-pep of BRMS1 PubMed: 30707944
Deposition date: 2019-01-21 Original release date: 2019-02-13
Authors: Neira, Jose Luis; Pantoja-Uceda, David
Citation: Pantoja-Uceda, David; Neira, Jose Luis; Contreras, Lellys; Manton, Christa; Welch, Danny; Rizzuti, Bruno. "The isolated C-terminal nuclear localization sequence of the breast cancer metastasis suppressor 1 is disordered" Arch. Biochem. Biophys. 664, 95-101 (2019).
Assembly members:
NLS2, polymer, 16 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41
Entity Sequences (FASTA):
NLS2: KARAAVSPQKRKSDGP
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 14 |
1H chemical shifts | 65 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NLS2 | 1 |
Entities:
Entity 1, NLS2 16 residues - Formula weight is not available
1 | LYS | ALA | ARG | ALA | ALA | VAL | SER | PRO | GLN | LYS | ||||
2 | ARG | LYS | SER | ASP | GLY | PRO |
Samples:
sample_1: NLS2 1.8 mM; ammonium acetate 50 mM; TSP 1%
sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
DOSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts