BMRB Entry 27761

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27761

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for ARNO Sec7
Deposition date:
2019-01-22
Original release date:
2020-02-28
Authors:
Etzkorn, Manuel; Viegas, Aldino; Yin, Dongsheng; Borggraefe, Jan; Viennet, Thibault; Falke, Marcel; Schmitz, Anton; Famulok, Michael
Citation:

Citation: Viegas, Aldino; Yin, Dongsheng; Borggraefe, Jan; Viennet, Thibault; Falke, Marcel; Schmitz, Anton; Famulok, Michael; Etzkorn, Manuel. "Molecular Architecture of a Network of Potential Intracellular EGFR Modulators: ARNO, CaM, Phospholipids, and the Juxtamembrane Segment"  Structure 28, 54-62.e5 (2020).
PubMed: 31780432

Assembly members:

Assembly members:
Sec7, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sec7: SETRNRKMAMGRKKFNMDPK KGIQFLVENELLQNTPEEIA RFLYKGEGLNKTAIGDYLGE REELNLAVLHAFVDLHEFTD LNLVQALRQFLWSFRLPGEA QKIDRMMEAFAQRYCLCNPG VFQSTDTCYVLSFAVIMLNT SLHNPNVRDKPGLERFVAMN RGINEGGDLPEELLRNLYDS IRNEPFKIP

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts150
1H chemical shifts150

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ARNO-Sec71

Entities:

Entity 1, ARNO-Sec7 189 residues - Formula weight is not available

Residues 58-60 represent a non-native tag

1   SERGLUTHRARGASNARGLYSMETALAMET
2   GLYARGLYSLYSPHEASNMETASPPROLYS
3   LYSGLYILEGLNPHELEUVALGLUASNGLU
4   LEULEUGLNASNTHRPROGLUGLUILEALA
5   ARGPHELEUTYRLYSGLYGLUGLYLEUASN
6   LYSTHRALAILEGLYASPTYRLEUGLYGLU
7   ARGGLUGLULEUASNLEUALAVALLEUHIS
8   ALAPHEVALASPLEUHISGLUPHETHRASP
9   LEUASNLEUVALGLNALALEUARGGLNPHE
10   LEUTRPSERPHEARGLEUPROGLYGLUALA
11   GLNLYSILEASPARGMETMETGLUALAPHE
12   ALAGLNARGTYRCYSLEUCYSASNPROGLY
13   VALPHEGLNSERTHRASPTHRCYSTYRVAL
14   LEUSERPHEALAVALILEMETLEUASNTHR
15   SERLEUHISASNPROASNVALARGASPLYS
16   PROGLYLEUGLUARGPHEVALALAMETASN
17   ARGGLYILEASNGLUGLYGLYASPLEUPRO
18   GLUGLULEULEUARGASNLEUTYRASPSER
19   ILEARGASNGLUPROPHELYSILEPRO

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks