BMRB Entry 27775
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27775
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: HLS3_Olduvai PubMed: 31264103
Deposition date: 2019-02-04 Original release date: 2019-07-02
Authors: Henen, Morkos; Schmitt, Lauren; Issaian, Aaron; Vogeli, Beat; Hansen, Kirk
Citation: Issaian, Aaron; Schmitt, Lauren; Born, Alexandra; Nichols, Parker; Sikela, James; Hansen, Kirk; Vogeli, Beat; Henen, Morkos. "Solution NMR backbone assignment reveals interaction-free tumbling of human lineage-specific Olduvai protein domains" Biomol. NMR Assign. 13, 339-343 (2019).
Assembly members:
HLS3, polymer, 94 residues, 10553.3948 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P
Entity Sequences (FASTA):
HLS3: SAAAASSASLEVDRIKKDQE
EEEDQGPPCPRLSRELLEVV
EPEVLQDSLDRCYSTPSSCL
EQPDSCQPYGSSFYALEEKH
VGFSLDLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 188 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 64 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HLS3 | 1 |
Entities:
Entity 1, HLS3 94 residues - 10553.3948 Da.
| 1 | SER | ALA | ALA | ALA | ALA | SER | SER | ALA | SER | LEU | ||||
| 2 | GLU | VAL | ASP | ARG | ILE | LYS | LYS | ASP | GLN | GLU | ||||
| 3 | GLU | GLU | GLU | ASP | GLN | GLY | PRO | PRO | CYS | PRO | ||||
| 4 | ARG | LEU | SER | ARG | GLU | LEU | LEU | GLU | VAL | VAL | ||||
| 5 | GLU | PRO | GLU | VAL | LEU | GLN | ASP | SER | LEU | ASP | ||||
| 6 | ARG | CYS | TYR | SER | THR | PRO | SER | SER | CYS | LEU | ||||
| 7 | GLU | GLN | PRO | ASP | SER | CYS | GLN | PRO | TYR | GLY | ||||
| 8 | SER | SER | PHE | TYR | ALA | LEU | GLU | GLU | LYS | HIS | ||||
| 9 | VAL | GLY | PHE | SER | LEU | ASP | LEU | GLU | HIS | HIS | ||||
| 10 | HIS | HIS | HIS | HIS |
Samples:
sample_1: HLS3, [U-100% 13C; U-100% 15N], 140 uM; sodium chloride 50 mM; sodium phosphate 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| Expt_3 (H[N[co[{CA|ca[C]}]]]) | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr_Analysis v2.4, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, data analysis, processing
NMR spectrometers:
- Varian INOVA 900 MHz
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts