BMRB Entry 27787

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27787

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Title:
Backbone (1H, 15N, 13C) chemical shift assignments for the CALX-CBD1 domain in the Ca2+-bound state
Deposition date:
2019-02-14
Original release date:
2020-06-18
Authors:
Kopke Salinas, Roberto; Abiko, Layara; Vitale, Phelipe
Citation:

Citation: Cardoso, Marcus; Rivera, J.; Vitale, Phelipe; Degenhardt, Maximilia; Abiko, Layara; Oliveira, Cristiano; Kopke Salinas, Roberto. "CALX-CBD1 Ca2+-binding cooperativity studied by NMR spectroscopy and ITC with Bayesian statistics"  Biophys. J. 119, 337-348 (2020).
PubMed: 32574558

Assembly members:

Assembly members:
CALX-CBD1, polymer, 131 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CALX-CBD1: MHHHHHHLEDDEEADDPIRM YFEPGHYTVMENCGEFEVRV VRRGDISTYASVEYETQDGT ASAGTDFVGRKGLLSFPPGV DEQRFRIEVIDDDVFEEDEC FYIRLFNPSEGVKLAVPMIA TVMILDDDHAG

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts106
1H chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CALX-CBD11
2Ca(2+), 12
3Ca(2+), 22
4Ca(2+), 32
5Ca(2+), 42

Entities:

Entity 1, CALX-CBD1 131 residues - Formula weight is not available

The segment between M425 and E433 corresponds to a 6His tag for purification. The amino acid sequence of the CALX-CBD1 starts at D434.

1   METHISHISHISHISHISHISLEUGLUASP
2   ASPGLUGLUALAASPASPPROILEARGMET
3   TYRPHEGLUPROGLYHISTYRTHRVALMET
4   GLUASNCYSGLYGLUPHEGLUVALARGVAL
5   VALARGARGGLYASPILESERTHRTYRALA
6   SERVALGLUTYRGLUTHRGLNASPGLYTHR
7   ALASERALAGLYTHRASPPHEVALGLYARG
8   LYSGLYLEULEUSERPHEPROPROGLYVAL
9   ASPGLUGLNARGPHEARGILEGLUVALILE
10   ASPASPASPVALPHEGLUGLUASPGLUCYS
11   PHETYRILEARGLEUPHEASNPROSERGLU
12   GLYVALLYSLEUALAVALPROMETILEALA
13   THRVALMETILELEUASPASPASPHISALA
14   GLY

Entity 2, Ca(2+), 1 - Ca - 40.078 Da.

1   CA

Related Database Links:

UNP O18367
AlphaFold O18367

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks