BMRB Entry 27811
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27811
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Elongation factor P from E.coli PubMed: 31178848
Deposition date: 2019-02-28 Original release date: 2019-06-26
Authors: Jagtap, Pravin Kumar Ankush; Macosek, Jakub; Hennig, Janosch
Citation: Volkwein, Wolfram; Krafczyk, Ralph; Jagtap, Pravin Kumar Ankush; Parr, Marina; Mankina, Elena; Macosek, Jakub; Guo, Zhenghuan; Furst, Maximilian Josef; Pfab, Miriam; Frishman, Dmitrij; Hennig, Janosch; Jung, Kirsten; Lassak, Jurgen. "Switching the Post-translational Modification of Translation Elongation Factor EF-P." Front. Microbiol. 10, 1148-1148 (2019).
Assembly members:
EFP, polymer, 188 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: NA
Entity Sequences (FASTA):
EFP: MATYYSNDFRAGLKIMLDGE
PYAVEASEFVKPGKGQAFAR
VKLRRLLTGTRVEKTFKSTD
SAEGADVVDMNLTYLYNDGE
FWHFMNNETFEQLSADAKAI
GDNAKWLLDQAECIVTLWNG
QPISVTPPNFVELEIVDTDP
GLKGDTAGTGGKPATLSTGA
VVKVPLFVQIGEVIKVDTRS
GEYVSRVK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 299 |
| 15N chemical shifts | 168 |
| 1H chemical shifts | 168 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EFP | 1 |
Entities:
Entity 1, EFP 188 residues - Formula weight is not available
| 1 | MET | ALA | THR | TYR | TYR | SER | ASN | ASP | PHE | ARG | ||||
| 2 | ALA | GLY | LEU | LYS | ILE | MET | LEU | ASP | GLY | GLU | ||||
| 3 | PRO | TYR | ALA | VAL | GLU | ALA | SER | GLU | PHE | VAL | ||||
| 4 | LYS | PRO | GLY | LYS | GLY | GLN | ALA | PHE | ALA | ARG | ||||
| 5 | VAL | LYS | LEU | ARG | ARG | LEU | LEU | THR | GLY | THR | ||||
| 6 | ARG | VAL | GLU | LYS | THR | PHE | LYS | SER | THR | ASP | ||||
| 7 | SER | ALA | GLU | GLY | ALA | ASP | VAL | VAL | ASP | MET | ||||
| 8 | ASN | LEU | THR | TYR | LEU | TYR | ASN | ASP | GLY | GLU | ||||
| 9 | PHE | TRP | HIS | PHE | MET | ASN | ASN | GLU | THR | PHE | ||||
| 10 | GLU | GLN | LEU | SER | ALA | ASP | ALA | LYS | ALA | ILE | ||||
| 11 | GLY | ASP | ASN | ALA | LYS | TRP | LEU | LEU | ASP | GLN | ||||
| 12 | ALA | GLU | CYS | ILE | VAL | THR | LEU | TRP | ASN | GLY | ||||
| 13 | GLN | PRO | ILE | SER | VAL | THR | PRO | PRO | ASN | PHE | ||||
| 14 | VAL | GLU | LEU | GLU | ILE | VAL | ASP | THR | ASP | PRO | ||||
| 15 | GLY | LEU | LYS | GLY | ASP | THR | ALA | GLY | THR | GLY | ||||
| 16 | GLY | LYS | PRO | ALA | THR | LEU | SER | THR | GLY | ALA | ||||
| 17 | VAL | VAL | LYS | VAL | PRO | LEU | PHE | VAL | GLN | ILE | ||||
| 18 | GLY | GLU | VAL | ILE | LYS | VAL | ASP | THR | ARG | SER | ||||
| 19 | GLY | GLU | TYR | VAL | SER | ARG | VAL | LYS |
Samples:
sample_1: EFP, [U-100% 13C; U-100% 15N], 0.8 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
Cara, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts