BMRB Entry 27819

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27819

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments of ITSN1 SH3A_L
Deposition date:
2019-03-04
Original release date:
2019-03-15
Authors:
Gerth, Fabian; Jaepel, Maria; Sticht, Jana; Schmitt, Xiao; Kuropka, Benno; Driller, Jan; Loll, Bernhard; Wahl, Markus; Pagel, Kevin; Haucke, Volker; Freund, Christian
Citation:

Citation: Gerth, Fabian; Jaepel, Maria; Sticht, Jana; Schmitt, Xiao; Kuropka, Benno; Driller, Jan; Loll, Bernhard; Wahl, Markus; Pagel, Kevin; Haucke, Volker; Freund, Christian. "Exon Inclusion Modulates Conformational Plasticity and Autoinhibition of the Intersectin 1 SH3A Domain."  Structure 27, 977-987 (2019).
PubMed: 31031201

Assembly members:

Assembly members:
ITSN1_SH3A-L, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ITSN1_SH3A-L: GSHMKVVYYRALYPFESRSH DEITIQPGDIVMVKGEWVDE SQTGEPGWLGGELKGKTGWF PANYAEKIPENEVPAPVKPV TDSTSAPAPKLALRETPAPL AVTS

Data sets:
Data typeCount
13C chemical shifts60
15N chemical shifts51
1H chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3A-L1

Entities:

Entity 1, SH3A-L 104 residues - Formula weight is not available

This is the long splice isoform of the SH3A domain with C-terminal extension, residues GSHM at the N-terminus are non-native.

1   GLYSERHISMETLYSVALVALTYRTYRARG
2   ALALEUTYRPROPHEGLUSERARGSERHIS
3   ASPGLUILETHRILEGLNPROGLYASPILE
4   VALMETVALLYSGLYGLUTRPVALASPGLU
5   SERGLNTHRGLYGLUPROGLYTRPLEUGLY
6   GLYGLULEULYSGLYLYSTHRGLYTRPPHE
7   PROALAASNTYRALAGLULYSILEPROGLU
8   ASNGLUVALPROALAPROVALLYSPROVAL
9   THRASPSERTHRSERALAPROALAPROLYS
10   LEUALALEUARGGLUTHRPROALAPROLEU
11   ALAVALTHRSER

Related Database Links:

UNP Q15811-1
AlphaFold Q9UQ92

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks