BMRB Entry 27832
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27832
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Title: Methyl Chemical shift Assignment of EIC from the thermophile thermoanaerobacter tengcongenesis PubMed: 31025174
Deposition date: 2019-03-07 Original release date: 2019-05-22
Authors: Dotas, Rochelle; Venditti, Vincenzo
Citation: Dotas, Rochelle; Venditti, Vincenzo. "Resonance assignment of the 128 kDa enzyme I dimer from Thermoanaerobacter tengcongensis" Biomol. NMR Assignments 13, 287-293 (2019).
Assembly members:
tEIC_methyls, polymer, 314 residues, Formula weight is not available
Natural source: Common Name: Caldanaerobacter subterraneus Taxonomy ID: 911092 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermoanaerobacter tengcongenesis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet21A
Entity Sequences (FASTA):
tEIC_methyls: MAETPDGKKVMLAANIGTPK
DVASALANGAEGVGLFRTEF
LYMDRNSLPSEEEQFEAYKE
VVEKMGGRPVTIRTLDIIGD
KELPYLDMPKEMNPFLGYRA
IRLCLDRPDIFKTQLRAILR
ASAYGNVQIMYPMISSVEEV
RKANSILEEVKAELDREGVK
YDKEIKVGIMVEIPSAAVTA
DILAKEVDFFSIGTNDLTQY
TLAVDRMNEHVKEYYQPFHP
AILRLVKMVIDAAHKEGKFA
AMCGEMAGDPLAAVILLGLG
LDEFSMSATSIPEIKNIIRN
VEYEKAKEIAEKALNMSEAR
EIEKMMKDVIKDIG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 94 |
| 1H chemical shifts | 282 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tEIC methyls | 1 |
Entities:
Entity 1, tEIC methyls 314 residues - Formula weight is not available
| 1 | MET | ALA | GLU | THR | PRO | ASP | GLY | LYS | LYS | VAL | ||||
| 2 | MET | LEU | ALA | ALA | ASN | ILE | GLY | THR | PRO | LYS | ||||
| 3 | ASP | VAL | ALA | SER | ALA | LEU | ALA | ASN | GLY | ALA | ||||
| 4 | GLU | GLY | VAL | GLY | LEU | PHE | ARG | THR | GLU | PHE | ||||
| 5 | LEU | TYR | MET | ASP | ARG | ASN | SER | LEU | PRO | SER | ||||
| 6 | GLU | GLU | GLU | GLN | PHE | GLU | ALA | TYR | LYS | GLU | ||||
| 7 | VAL | VAL | GLU | LYS | MET | GLY | GLY | ARG | PRO | VAL | ||||
| 8 | THR | ILE | ARG | THR | LEU | ASP | ILE | ILE | GLY | ASP | ||||
| 9 | LYS | GLU | LEU | PRO | TYR | LEU | ASP | MET | PRO | LYS | ||||
| 10 | GLU | MET | ASN | PRO | PHE | LEU | GLY | TYR | ARG | ALA | ||||
| 11 | ILE | ARG | LEU | CYS | LEU | ASP | ARG | PRO | ASP | ILE | ||||
| 12 | PHE | LYS | THR | GLN | LEU | ARG | ALA | ILE | LEU | ARG | ||||
| 13 | ALA | SER | ALA | TYR | GLY | ASN | VAL | GLN | ILE | MET | ||||
| 14 | TYR | PRO | MET | ILE | SER | SER | VAL | GLU | GLU | VAL | ||||
| 15 | ARG | LYS | ALA | ASN | SER | ILE | LEU | GLU | GLU | VAL | ||||
| 16 | LYS | ALA | GLU | LEU | ASP | ARG | GLU | GLY | VAL | LYS | ||||
| 17 | TYR | ASP | LYS | GLU | ILE | LYS | VAL | GLY | ILE | MET | ||||
| 18 | VAL | GLU | ILE | PRO | SER | ALA | ALA | VAL | THR | ALA | ||||
| 19 | ASP | ILE | LEU | ALA | LYS | GLU | VAL | ASP | PHE | PHE | ||||
| 20 | SER | ILE | GLY | THR | ASN | ASP | LEU | THR | GLN | TYR | ||||
| 21 | THR | LEU | ALA | VAL | ASP | ARG | MET | ASN | GLU | HIS | ||||
| 22 | VAL | LYS | GLU | TYR | TYR | GLN | PRO | PHE | HIS | PRO | ||||
| 23 | ALA | ILE | LEU | ARG | LEU | VAL | LYS | MET | VAL | ILE | ||||
| 24 | ASP | ALA | ALA | HIS | LYS | GLU | GLY | LYS | PHE | ALA | ||||
| 25 | ALA | MET | CYS | GLY | GLU | MET | ALA | GLY | ASP | PRO | ||||
| 26 | LEU | ALA | ALA | VAL | ILE | LEU | LEU | GLY | LEU | GLY | ||||
| 27 | LEU | ASP | GLU | PHE | SER | MET | SER | ALA | THR | SER | ||||
| 28 | ILE | PRO | GLU | ILE | LYS | ASN | ILE | ILE | ARG | ASN | ||||
| 29 | VAL | GLU | TYR | GLU | LYS | ALA | LYS | GLU | ILE | ALA | ||||
| 30 | GLU | LYS | ALA | LEU | ASN | MET | SER | GLU | ALA | ARG | ||||
| 31 | GLU | ILE | GLU | LYS | MET | MET | LYS | ASP | VAL | ILE | ||||
| 32 | LYS | ASP | ILE | GLY |
Samples:
sample_1: tEIC methyls, [U-2H; U-13C, methyl], 1 mM; DTT 2 mM; TRIS 20 mM; EDTA 1 mM; MgCl2 4 mM; NaCl 100 mM; D2O 10%
sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - data analysis
NMR spectrometers:
- Bruker Avance 700 MHz