BMRB Entry 27838

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27838

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Title:
Backbone 1H, 13C and 15N chemical shift assignment of the cyclase IdmH from the indanomycin NRPS/PKS from Streptomyces antibioticus
Deposition date:
2019-03-19
Original release date:
2021-07-21
Authors:
Kalverda, Arnout; Drulyte, Ieva; Berry, Alan
Citation:

Citation: Drulyte, Ieva; Obajdin, Jana; Trinh, Chi; Kalverda, Arnout; van der Kamp, Marc; Hemsworth, Glyn; Berry, Alan. "Crystal structure of the putative cyclase IdmH from the indanomycin nonribosomal peptide synthase/polyketide synthase"  IUCrJ 6, 1120-1133 (2019).
PubMed: 31709067

Assembly members:

Assembly members:
IdmH, polymer, 164 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptomyces antibioticus   Taxonomy ID: 1890   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces antibioticus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28(a)+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IdmH: GSSHHHHHHSSGLVPRGSHM AHQPSDTIAGLYEAFNSGDL ETLRELIAPDAVIHLPGTAG DAEHPPGTPRDREGWLGVWQ FTQAFFPDMTATVQDIVQTG DLVATRCVARGTHSIEFMGV PPTGRPFEMTMLNMSRVRDG RIVEHWTISDNVTMLAQLGV KASL

Data sets:
Data typeCount
13C chemical shifts408
15N chemical shifts125
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IdmH 11
2IdmH 21

Entities:

Entity 1, IdmH 1 164 residues - Formula weight is not available

Residues 1-19 are a his tag

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   ALAHISGLNPROSERASPTHRILEALAGLY
4   LEUTYRGLUALAPHEASNSERGLYASPLEU
5   GLUTHRLEUARGGLULEUILEALAPROASP
6   ALAVALILEHISLEUPROGLYTHRALAGLY
7   ASPALAGLUHISPROPROGLYTHRPROARG
8   ASPARGGLUGLYTRPLEUGLYVALTRPGLN
9   PHETHRGLNALAPHEPHEPROASPMETTHR
10   ALATHRVALGLNASPILEVALGLNTHRGLY
11   ASPLEUVALALATHRARGCYSVALALAARG
12   GLYTHRHISSERILEGLUPHEMETGLYVAL
13   PROPROTHRGLYARGPROPHEGLUMETTHR
14   METLEUASNMETSERARGVALARGASPGLY
15   ARGILEVALGLUHISTRPTHRILESERASP
16   ASNVALTHRMETLEUALAGLNLEUGLYVAL
17   LYSALASERLEU

Related Database Links:

UNP ACN69984.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks