BMRB Entry 27839
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27839
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Title: Iga1 Protease G5 domain PubMed: 31390088
Deposition date: 2019-03-19 Original release date: 2019-04-24
Authors: Eisenmesser, Elan; Chi, Ying-Chih Chi; Paukovich, Natasia; Redzic, Jasmina; Rahkola, Jeremy; Janoff, Edward
Citation: Paukovich, Natasia; Redzic, Jasmina; Chi, Ying-Chih; Rahkola, Jeremy; Issaian, Aaron; Blue, Ashley; Hansen, Kirk; Janoff, Edward; Eisenmesser, Elan. "Streptococcus pneumoniae G5 domains bind different ligands" Protein Sci. 28, 1797-1805 (2019).
Assembly members:
IgA1P-G5, polymer, 84 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pj401
Entity Sequences (FASTA):
IgA1P-G5: GSHNKPELLYREETIETKID
FQEEIQENPDLAEGTVRVKQ
EGKLGKKVEIVRIFSVNKEE
VSREIVSTSTTAPSPRIVEK
GTKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 329 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 461 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IgA1 protease G5 monomer | 1 |
Entities:
Entity 1, IgA1 protease G5 monomer 84 residues - Formula weight is not available
| 1 | GLY | SER | HIS | ASN | LYS | PRO | GLU | LEU | LEU | TYR | ||||
| 2 | ARG | GLU | GLU | THR | ILE | GLU | THR | LYS | ILE | ASP | ||||
| 3 | PHE | GLN | GLU | GLU | ILE | GLN | GLU | ASN | PRO | ASP | ||||
| 4 | LEU | ALA | GLU | GLY | THR | VAL | ARG | VAL | LYS | GLN | ||||
| 5 | GLU | GLY | LYS | LEU | GLY | LYS | LYS | VAL | GLU | ILE | ||||
| 6 | VAL | ARG | ILE | PHE | SER | VAL | ASN | LYS | GLU | GLU | ||||
| 7 | VAL | SER | ARG | GLU | ILE | VAL | SER | THR | SER | THR | ||||
| 8 | THR | ALA | PRO | SER | PRO | ARG | ILE | VAL | GLU | LYS | ||||
| 9 | GLY | THR | LYS | LYS |
Samples:
sample_1: IgA1P-G5, [U-13C; U-15N; U-2H], 0.5 mM; MES 50 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 25 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian DRX 900 MHz
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts