BMRB Entry 27850
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27850
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Title: ENDD G5 domain PubMed: 31390088
Deposition date: 2019-03-22 Original release date: 2019-04-24
Authors: Eisenmesser, Elan; Chi, Ying-Chih; Paukovich, Natasia; Redzic, Jasmina; Rahkola, Jeremy; Janoff, Edward
Citation: Paukovich, Natasia; Redzic, Jasmina; Chi, Ying-Chih; Rahkola, Jeremy; Issaian, Aaron; Blue, Ashley; Hansen, Kirk; Janoff, Edward; Eisenmesser, Elan. "Streptococcus pneumoniae G5 domains bind different ligands" Protein Sci. 28, 1797-1805 (2019).
Assembly members:
ENDD_G5_domain, polymer, 81 residues, Formula weight is not available
Natural source: Common Name: Streptococcus pneumoniae Taxonomy ID: 1313 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus pneumoniae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pj401
Entity Sequences (FASTA):
ENDD_G5_domain: GSHSDQQKAPKLEVQEEKVA
FHRQEHENAEMLVGEQRVII
QGRDGLLRHVFEVDENGQRR
LRSTEVIQEAIPEIVEIGTK
V
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 213 |
15N chemical shifts | 69 |
1H chemical shifts | 69 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ENDD G5 domain | 1 |
Entities:
Entity 1, ENDD G5 domain 81 residues - Formula weight is not available
1 | GLY | SER | HIS | SER | ASP | GLN | GLN | LYS | ALA | PRO | ||||
2 | LYS | LEU | GLU | VAL | GLN | GLU | GLU | LYS | VAL | ALA | ||||
3 | PHE | HIS | ARG | GLN | GLU | HIS | GLU | ASN | ALA | GLU | ||||
4 | MET | LEU | VAL | GLY | GLU | GLN | ARG | VAL | ILE | ILE | ||||
5 | GLN | GLY | ARG | ASP | GLY | LEU | LEU | ARG | HIS | VAL | ||||
6 | PHE | GLU | VAL | ASP | GLU | ASN | GLY | GLN | ARG | ARG | ||||
7 | LEU | ARG | SER | THR | GLU | VAL | ILE | GLN | GLU | ALA | ||||
8 | ILE | PRO | GLU | ILE | VAL | GLU | ILE | GLY | THR | LYS | ||||
9 | VAL |
Samples:
sample_1: ENDD G5 domain, [U-13C; U-15N; U-2H], 0.420 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts