BMRB Entry 27854

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27854

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Human DC-SIGN Carbohydrate Recognition Domain
Deposition date:
2019-03-26
Original release date:
2019-07-18
Authors:
Valverde, Pablo; Delgado, Sandra; Martinez, J.Daniel; Jimenez-Barbero, Jesus; Arda, Ana
Citation:

Citation: Valverde, Pablo; Delgado, Sandra; Martinez, J.Daniel; Vendeville, Jean-Baptiste; Malassis, Julien; Linclau, Bruno; Reichardt, Niels-Christian; Canada, F.Javier; Jimenez-Barbero, Jesus; Arda, Ana. "Molecular Insights into DC-SIGN Binding to Self-Antigens: The Interaction with the Blood Group A/B Antigens"  ACS Chem Biol. 14, 1660-1671 (2019).
PubMed: 31283166

Assembly members:

Assembly members:
DC-SIGN_CRD, polymer, 177 residues, 20097 Da.
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DC-SIGN_CRD: MGSSHHHHHHSSGLVPRGSH MAERLCHPCPWEWTFFQGNC YFMSNSQRNWHDSITACKEV GAQLVVIKSAEEQNFLQLQS SRSNRFTWMGLSDLNQEGTW QWVDGSPLLPSFKQYWNRGE PNNVGEEDCAEFSGNGWNDD KCNLAKFWICKKSAASCSRD EEQFLSPAPATPNPPPA

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts232
1H chemical shifts232

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DC-SIGN CRD1
2Calcium ion2

Entities:

Entity 1, DC-SIGN CRD 177 residues - 20097 Da.

Residues 1-26 (228-253) represent a non-native affinity tag. Residues 27-177 correspond to residues 254-404 in PDB 1SL5 (CRD domain).

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALAGLUARGLEUCYSHISPROCYSPRO
4   TRPGLUTRPTHRPHEPHEGLNGLYASNCYS
5   TYRPHEMETSERASNSERGLNARGASNTRP
6   HISASPSERILETHRALACYSLYSGLUVAL
7   GLYALAGLNLEUVALVALILELYSSERALA
8   GLUGLUGLNASNPHELEUGLNLEUGLNSER
9   SERARGSERASNARGPHETHRTRPMETGLY
10   LEUSERASPLEUASNGLNGLUGLYTHRTRP
11   GLNTRPVALASPGLYSERPROLEULEUPRO
12   SERPHELYSGLNTYRTRPASNARGGLYGLU
13   PROASNASNVALGLYGLUGLUASPCYSALA
14   GLUPHESERGLYASNGLYTRPASNASPASP
15   LYSCYSASNLEUALALYSPHETRPILECYS
16   LYSLYSSERALAALASERCYSSERARGASP
17   GLUGLUGLNPHELEUSERPROALAPROALA
18   THRPROASNPROPROPROALA

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Related Database Links:

PDB 1SL4 1SL5 2IT5 2IT6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks