BMRB Entry 27857

Name: Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments of RbfA
Published: 2019-04-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27857

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments of RbfA PubMed: 32671633

Deposition date: 2019-03-29 Original release date: 2019-04-01

Authors: Schedlbauer, Andreas; Kaminishi, Tatsuya; Lopez-Alonso, Jorge; Iturrioz, Idoia; Ochoa Lizarralde, Borja; Gil-Carton, David; Fucini, Paola; Connell, Sean

Citation: Schedlbauer, Andreas; Iturrioz, Idoia; Ochoa-Lizarralde, Borja; Capuni, Retina; Han, Xu; de Astigarraga, Elisa; Diercks, Tammo; Fucini, Paola; Connell, Sean. "Backbone and sidechain NMR assignments for the ribosome maturation factor RbfA from Escherichia coli" Biomol. NMR Assign. 14, 317-321 (2020).

Assembly members:
RbfA, polymer, 140 residues, 15911.32 Da.

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: P7XC3GH

Entity Sequences (FASTA):
RbfA: SAKEFGRPQRVAQEMQKEIA LILQREIKDPRLGMMTTVSG VEMSRDLAYAKVYVTFLNDK DEDAVKAGIKALQEASGFIR SLLGKAMRLRIVPELTFFYD NSLVEGMRMSNLVTSVVKHD EERRVNPDDSKEDALEVLFQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	522
15N chemical shifts	114
1H chemical shifts	835

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RbfA	1

Entities:

Entity 1, RbfA 140 residues - 15911.32 Da.

1	SER	ALA	LYS	GLU	PHE	GLY	ARG	PRO	GLN	ARG
2	VAL	ALA	GLN	GLU	MET	GLN	LYS	GLU	ILE	ALA
3	LEU	ILE	LEU	GLN	ARG	GLU	ILE	LYS	ASP	PRO
4	ARG	LEU	GLY	MET	MET	THR	THR	VAL	SER	GLY
5	VAL	GLU	MET	SER	ARG	ASP	LEU	ALA	TYR	ALA
6	LYS	VAL	TYR	VAL	THR	PHE	LEU	ASN	ASP	LYS
7	ASP	GLU	ASP	ALA	VAL	LYS	ALA	GLY	ILE	LYS
8	ALA	LEU	GLN	GLU	ALA	SER	GLY	PHE	ILE	ARG
9	SER	LEU	LEU	GLY	LYS	ALA	MET	ARG	LEU	ARG
10	ILE	VAL	PRO	GLU	LEU	THR	PHE	PHE	TYR	ASP
11	ASN	SER	LEU	VAL	GLU	GLY	MET	ARG	MET	SER
12	ASN	LEU	VAL	THR	SER	VAL	VAL	LYS	HIS	ASP
13	GLU	GLU	ARG	ARG	VAL	ASN	PRO	ASP	ASP	SER
14	LYS	GLU	ASP	ALA	LEU	GLU	VAL	LEU	PHE	GLN

Samples:

sample_1: RbfA, [U-98% 13C; U-98% 15N], 400 uM; HEPES 10 mM; MgCl2 6 mM; NH4Cl 30 mM; TCEP 75 uM

sample_conditions_1: pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CNH NOESY	sample_1	isotropic	sample_conditions_1
3D HNH NOESY	sample_1	isotropic	sample_conditions_1
3D HCH NOESY	sample_1	isotropic	sample_conditions_1
3D HNCAHA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CAHA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v9.8, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

UNP	P0A7G2
AlphaFold	Q2M943

Biological Magnetic Resonance Data Bank