BMRB Entry 27862
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27862
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Title: NMR 1H chemical shift assignment heptapeptide EVNPAVP PubMed: 31182482
Deposition date: 2019-04-01 Original release date: 2019-05-30
Authors: Pichlo, Christian; Jutten, Linda; Wojtalla, Fabian; Schacherl, Magdalena; Diaz, Dolores; Baumann, Ulrich
Citation: Pichlo, Christian; Jutten, Linda; Wojtalla, Fabian; Schacherl, Magdalena; Diaz, Dolores; Baumann, Ulrich. "Molecular determinants of the mechanism and substrate specificity of Clostridium difficile proline-proline endopeptidase-1" J. Biol. Chem. 294, 11525-11535 (2019).
Assembly members:
EVNPAVP_heptapeptide, polymer, 7 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
EVNPAVP_heptapeptide: EVNPAVP
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 47 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | EVNPAVP heptapeptide | 1 |
Entities:
Entity 1, EVNPAVP heptapeptide 7 residues - Formula weight is not available
1 | GLU | VAL | ASN | PRO | ALA | VAL | PRO |
Samples:
sample_1: EVNPAVP 2 mM; PBS buffer 50 mM
sample_conditions_1: ionic strength: 0.175 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment, peak picking
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz