BMRB Entry 27865
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27865
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Title: Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin PubMed: 31776187
Deposition date: 2019-04-01 Original release date: 2019-11-25
Authors: Charoenwattanasatien, Ratana; Zinzius, Karen; Scholz, Martin; Wicke, Susann; Tanaka, Hideaki; Brandenburg, Johann; Ikegami, Takahisa; Matsumoto, Takashi; Oda, Takashi; Sato, Mamoru; Hippler, Michael; Kurisu, Genji
Citation: Charoenwattanasatien, Ratana; Zinzius, Karen; Scholz, Martin; Wicke, Susann; Tanaka, Hideaki; Brandenburg, Johann; Marchetti, Giulia; Ikegami, Takahisa; Matsumoto, Takashi; Oda, Takashi; Sato, Mamoru; Hippler, Michael; Kurisu, Genji. "Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin in the green alga" J. Biol. Chem. 295, 170-180 (2020).
Assembly members:
calredoxin, polymer, 356 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: green algae Taxonomy ID: 3055 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Chlamydomonas reinhardtii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b(+)
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 15 |
| 1H chemical shifts | 45 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calredoxin | 1 |
| 2 | Calcium, 1 | 2 |
| 3 | Calcium, 2 | 2 |
| 4 | Calcium, 3 | 2 |
| 5 | Calcium, 4 | 2 |
Entities:
Entity 1, calredoxin 356 residues - Formula weight is not available
| 1 | MET | CYS | SER | ALA | ARG | SER | VAL | ARG | VAL | VAL | ||||
| 2 | PRO | ARG | ALA | ALA | THR | PHE | GLU | GLY | LEU | MET | ||||
| 3 | ASP | ASP | ALA | SER | LYS | ALA | LYS | MET | GLU | GLU | ||||
| 4 | LEU | GLU | ARG | ARG | PHE | LYS | MET | ALA | ASP | VAL | ||||
| 5 | ASP | GLY | ASN | GLY | HIS | ILE | ASP | ARG | GLU | GLU | ||||
| 6 | LEU | ARG | ASN | LEU | LEU | GLU | SER | MET | GLU | SER | ||||
| 7 | GLY | GLU | VAL | TYR | MET | MET | SER | GLN | HIS | TRP | ||||
| 8 | LEU | PRO | GLU | ASP | GLU | LEU | GLU | ARG | CYS | MET | ||||
| 9 | GLU | GLN | TYR | ASP | VAL | ASN | LYS | ASP | GLY | VAL | ||||
| 10 | ILE | SER | PHE | GLU | GLU | PHE | LYS | GLN | ILE | ILE | ||||
| 11 | TYR | ASP | GLY | LEU | LEU | LEU | GLU | GLY | THR | LEU | ||||
| 12 | ALA | GLU | TYR | GLU | SER | ALA | PHE | LYS | ALA | VAL | ||||
| 13 | ASP | LYS | SER | GLY | ASN | GLY | THR | ILE | GLY | ALA | ||||
| 14 | THR | GLU | LEU | SER | LYS | LEU | PHE | ALA | SER | LEU | ||||
| 15 | GLY | ASN | PRO | VAL | SER | LEU | GLU | LYS | LEU | VAL | ||||
| 16 | ASP | LEU | MET | GLN | MET | TYR | ASP | LYS | ASP | ASP | ||||
| 17 | SER | GLY | GLN | ILE | GLU | PHE | PRO | GLU | PHE | LEU | ||||
| 18 | LEU | MET | PHE | ARG | ASN | SER | LEU | LEU | ASP | LEU | ||||
| 19 | LYS | ASP | MET | THR | THR | TYR | MET | THR | LEU | ASP | ||||
| 20 | GLU | ALA | GLY | ALA | GLY | SER | SER | GLY | SER | LEU | ||||
| 21 | VAL | ASP | ALA | VAL | GLU | GLY | ASP | MET | THR | LEU | ||||
| 22 | ILE | PHE | SER | GLU | GLU | GLU | LEU | ASP | ALA | LEU | ||||
| 23 | ILE | SER | ALA | ASN | PRO | ASP | LYS | LEU | VAL | VAL | ||||
| 24 | VAL | PHE | GLY | ALA | LEU | THR | TRP | CYS | ARG | PRO | ||||
| 25 | CYS | LYS | GLY | MET | GLN | ARG | PRO | VAL | GLN | LYS | ||||
| 26 | LEU | ALA | GLU | HIS | TYR | LYS | ASP | HIS | ILE | VAL | ||||
| 27 | PHE | VAL | LYS | LEU | PHE | GLY | ASN | ALA | ASN | LYS | ||||
| 28 | GLN | THR | LYS | ARG | ILE | PHE | LYS | GLU | ARG | PHE | ||||
| 29 | GLN | ILE | ARG | SER | THR | PRO | CYS | PHE | ILE | THR | ||||
| 30 | LEU | ARG | LYS | GLY | GLU | PRO | VAL | TYR | THR | GLN | ||||
| 31 | THR | GLY | SER | ASN | LYS | GLU | LYS | LEU | GLU | ALA | ||||
| 32 | GLY | LEU | ARG | SER | LEU | ILE | ALA | ASN | PRO | PRO | ||||
| 33 | VAL | GLY | MET | ILE | TYR | PRO | SER | ALA | GLU | ALA | ||||
| 34 | LEU | ALA | ALA | LEU | GLN | ASP | PRO | ASN | SER | SER | ||||
| 35 | SER | VAL | ASP | LYS | LEU | ALA | ALA | ALA | LEU | GLU | ||||
| 36 | HIS | HIS | HIS | HIS | HIS | HIS |
Entity 2, Calcium, 1 - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: calredoxin, [13C]-Met, 0.3 ± 0.03 mM; HEPES 20 ± 2 mM; sodium chloride 150 ± 15 mM; DTT 0.5 ± 0.05 mM; calcium chloride 2.5 ± 0.25 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5, Bruker Biospin - chemical shift assignment, collection, processing
NMR spectrometers:
- Bruker Avance 950 MHz