BMRB Entry 27869

Name: Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens
Published: 2019-07-09

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR27869

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens

Deposition date:

2019-04-04

Original release date:

2019-07-09

Authors:

Teixeira, Liliana; Portela, Pilar; Bruix, Marta; Salgueiro, Carlos

Citation:

Citation: Teixeira, Liliana; Portela, Pilar; Morgado, Leonor; Pantoja-Uceda, David; Bruix, Marta; Salgueiro, Carlos. "Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens" Biomol. NMR Assignments 13, 321-326 (2019).
PubMed: 31119489

Assembly members:

Assembly members:
PccH_Polypeptide, polymer, 129 residues, Formula weight is not available
entity_HEM, non-polymer, 616.487 Da.

Natural source:

Natural source: Common Name: Geobacter sulfurreducens Taxonomy ID: 35554 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacter sulfurreducens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCS3274

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PccH_Polypeptide: GEVTYRKDIKPIFDVRCAGC HGADAAPEYHAFKAEKEKWL AKGQGMRMDTYSHLIFYTAW PDTGALMRRLDDGKNSKDAK PGNMYRHLGATEEERQRNLA VFKAWVGVWNLKKWPDITKE ELNAITVTY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	485
15N chemical shifts	121
1H chemical shifts	679

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PccH_Polypeptide	1
2	PROTOPORPHYRIN IX CONTAINING FE	2

Entities:

Entity 1, PccH_Polypeptide 129 residues - Formula weight is not available

1

GLY

GLU

VAL

THR

TYR

ARG

LYS

ASP

ILE

LYS

2

PRO

ILE

PHE

ASP

VAL

ARG

CYS

ALA

GLY

CYS

3

HIS

GLY

ALA

ASP

ALA

PRO

GLU

TYR

HIS

4

ALA

PHE

LYS

ALA

GLU

LYS

GLU

LYS

TRP

LEU

5

ALA

LYS

GLY

GLN

GLY

MET

ARG

MET

ASP

THR

6

TYR

SER

HIS

LEU

ILE

PHE

TYR

THR

ALA

TRP

7

PRO

ASP

THR

GLY

ALA

LEU

MET

ARG

LEU

8

ASP

GLY

LYS

ASN

SER

LYS

ASP

ALA

LYS

9

PRO

GLY

ASN

MET

TYR

ARG

HIS

LEU

GLY

ALA

10

THR

GLU

ARG

GLN

ARG

ASN

LEU

ALA

11

VAL

PHE

LYS

ALA

TRP

VAL

GLY

VAL

TRP

ASN

12

LEU

LYS

TRP

PRO

ASP

ILE

THR

LYS

GLU

13

GLU

LEU

ASN

ALA

ILE

THR

VAL

THR

TYR

Entity 2, PROTOPORPHYRIN IX CONTAINING FE - C34 H32 Fe N4 O4 - 616.487 Da.

1

HEM

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_2
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 27869

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: