BMRB Entry 27876

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27876

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Title:
NMR resonance assignment for the GSPII-C domain of PilF from Thermus thermophilus in complex with c-di-GMP
Deposition date:
2019-04-10
Original release date:
2019-08-14
Authors:
Keller, Heiko; Duchardt-Ferner, Elke; Woehnert, Jens
Citation:

Citation: Keller, Heiko; Kruse, Kerstin; Averhoff, Beate; Duchardt-Ferner, Elke; Woehnert, Jens. "NMR resonance assignments for the GSPII-C domain of the PilF ATPase from Thermus thermophilus in complex with c-di-GMP"  Biomol. NMR Assignments 13, 361-366 (2019).
PubMed: 31372934

Assembly members:

Assembly members:
PilF_294-482, polymer, 191 residues, 21223 Da.
entity_C2E, non-polymer, 690.411 Da.

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Thermus thermophilus   Vector: pET-11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PilF_294-482: GSLFRRAYPQKNRLGEVLVQ EGKLSREALKEALEVQKGLP RAKPLGEILVELGLARPEDV EEALQKQRRGGGRLEDTLVQ SGKLRPEALAQAVATQLGYP YVDPEEDPPDPGAPLLLPED LCRRYGVFPHRLEGNRLVLL MKDPRNILALDDVRLALKRK GLNYEVAPAVATEAAITKLI ERFYGKAELSE

Data typeCount
13C chemical shifts858
15N chemical shifts178
1H chemical shifts1370

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PilF 294-4821
2c-di-GMP2

Entities:

Entity 1, PilF 294-482 191 residues - 21223 Da.

Residues 1-2 represent a non-native tag remaining from TEV cleavage.

1   GLYSERLEUPHEARGARGALATYRPROGLN
2   LYSASNARGLEUGLYGLUVALLEUVALGLN
3   GLUGLYLYSLEUSERARGGLUALALEULYS
4   GLUALALEUGLUVALGLNLYSGLYLEUPRO
5   ARGALALYSPROLEUGLYGLUILELEUVAL
6   GLULEUGLYLEUALAARGPROGLUASPVAL
7   GLUGLUALALEUGLNLYSGLNARGARGGLY
8   GLYGLYARGLEUGLUASPTHRLEUVALGLN
9   SERGLYLYSLEUARGPROGLUALALEUALA
10   GLNALAVALALATHRGLNLEUGLYTYRPRO
11   TYRVALASPPROGLUGLUASPPROPROASP
12   PROGLYALAPROLEULEULEUPROGLUASP
13   LEUCYSARGARGTYRGLYVALPHEPROHIS
14   ARGLEUGLUGLYASNARGLEUVALLEULEU
15   METLYSASPPROARGASNILELEUALALEU
16   ASPASPVALARGLEUALALEULYSARGLYS
17   GLYLEUASNTYRGLUVALALAPROALAVAL
18   ALATHRGLUALAALAILETHRLYSLEUILE
19   GLUARGPHETYRGLYLYSALAGLULEUSER
20   GLU

Entity 2, c-di-GMP - C20 H24 N10 O14 P2 - 690.411 Da.

1   C2E

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