BMRB Entry 27886

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27886

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Shaker-VSD
Deposition date:
2019-04-30
Original release date:
2019-05-30
Authors:
Chen, Hongbo; Chanda, Baron; Henzler-Wildman, Katherine
Citation:

Citation: Chen, Hongbo; Pan, Junkun; Gandhi, Disha; Dockendorff, Christopher; Cui, Qiang; Chanda, Baron; Henzler-Wildman, Katherine. "NMR Structural Analysis of Isolated Shaker Voltage-Sensing Domain in LPPG Micelles"  Biophys. J. 117, 388-398 (2019).
PubMed: 31301804

Assembly members:

Assembly members:
Shaker-VSD, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Shaker-VSD: GGGGFEYPESSQAARVVAII SVFVILLSIVIFCLETLPEF KHYKVFNTTTNGTKIEEDEV PDITDPFFLIETLCIIWFTF ELTVRFLACPNKLNFCRDVM NVIDIIAIIPYFITLATVVA EEEDTLNLPKAPVSPQDKSS NQAMSLAILRVIRLVRVFRI FKLSRHSKGLQILGRTLKAS MRELG

Data sets:
Data typeCount
13C chemical shifts524
15N chemical shifts163
1H chemical shifts178

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Shaker-VSD1

Entities:

Entity 1, Shaker-VSD 185 residues - Formula weight is not available

G213, G214, G215, G216 (first four) are resides left after TEV protease cleavage and do not belong to Shaker-VSD.

1   GLYGLYGLYGLYPHEGLUTYRPROGLUSER
2   SERGLNALAALAARGVALVALALAILEILE
3   SERVALPHEVALILELEULEUSERILEVAL
4   ILEPHECYSLEUGLUTHRLEUPROGLUPHE
5   LYSHISTYRLYSVALPHEASNTHRTHRTHR
6   ASNGLYTHRLYSILEGLUGLUASPGLUVAL
7   PROASPILETHRASPPROPHEPHELEUILE
8   GLUTHRLEUCYSILEILETRPPHETHRPHE
9   GLULEUTHRVALARGPHELEUALACYSPRO
10   ASNLYSLEUASNPHECYSARGASPVALMET
11   ASNVALILEASPILEILEALAILEILEPRO
12   TYRPHEILETHRLEUALATHRVALVALALA
13   GLUGLUGLUASPTHRLEUASNLEUPROLYS
14   ALAPROVALSERPROGLNASPLYSSERSER
15   ASNGLNALAMETSERLEUALAILELEUARG
16   VALILEARGLEUVALARGVALPHEARGILE
17   PHELYSLEUSERARGHISSERLYSGLYLEU
18   GLNILELEUGLYARGTHRLEULYSALASER
19   METARGGLULEUGLY

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SPARKY: Backbone or all simulated peaks