BMRB Entry 27894
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27894
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Title: Backbone assignment of human FOXO1 PubMed: 31913281
Deposition date: 2019-05-08 Original release date: 2020-02-26
Authors: Hennig, Janosch; Hollmann, Nele Merret
Citation: Ibarra, Ignacio; Hollmann, Nele Merret; Klaus, Bernd; Augsten, Sandra; Velten, Britta; Hennig, Janosch; Zaugg, Judith. "Mechanistic insights into transcription factor cooperativity and its impact on protein-phenotype interactions" Nat. Commun. 11, 124-124 (2020).
Assembly members:
FOXO1, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-22
Entity Sequences (FASTA):
FOXO1: GPGPLAGQPRKSSSSRRNAW
GNLSYADLITKAIESSAEKR
LTLSQIYEWMVKSVPYFKDK
GDSNSSAGWKNSIRHNLSLH
SKFIRVQNEGTGKSSWWMLN
PEGGKSGKSPRRRAASMDNN
SKFAKSRSRA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 198 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 106 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FOXO1 | 1 |
Entities:
Entity 1, FOXO1 130 residues - Formula weight is not available
| 1 | GLY | PRO | GLY | PRO | LEU | ALA | GLY | GLN | PRO | ARG | |
| 2 | LYS | SER | SER | SER | SER | ARG | ARG | ASN | ALA | TRP | |
| 3 | GLY | ASN | LEU | SER | TYR | ALA | ASP | LEU | ILE | THR | |
| 4 | LYS | ALA | ILE | GLU | SER | SER | ALA | GLU | LYS | ARG | |
| 5 | LEU | THR | LEU | SER | GLN | ILE | TYR | GLU | TRP | MET | |
| 6 | VAL | LYS | SER | VAL | PRO | TYR | PHE | LYS | ASP | LYS | |
| 7 | GLY | ASP | SER | ASN | SER | SER | ALA | GLY | TRP | LYS | |
| 8 | ASN | SER | ILE | ARG | HIS | ASN | LEU | SER | LEU | HIS | |
| 9 | SER | LYS | PHE | ILE | ARG | VAL | GLN | ASN | GLU | GLY | |
| 10 | THR | GLY | LYS | SER | SER | TRP | TRP | MET | LEU | ASN | |
| 11 | PRO | GLU | GLY | GLY | LYS | SER | GLY | LYS | SER | PRO | |
| 12 | ARG | ARG | ARG | ALA | ALA | SER | MET | ASP | ASN | ASN | |
| 13 | SER | LYS | PHE | ALA | LYS | SER | ARG | SER | ARG | ALA |
Samples:
sample_1: FOXO1, [U-99% 13C; U-99% 15N], 0.25 mM; D2O, [U-99% 2H], 10%; bis-TRIS 50 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Fred Damberger - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts