BMRB Entry 27902

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27902

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Title:
S64V-EmrE assignments
Deposition date:
2019-05-13
Original release date:
2021-07-28
Authors:
Henzler-Wildman, Katherine; Wu, Chao
Citation:

Citation: Shcherbakov, Alexander; Hisao, Grant; Mandala, Venkata; Thomas, Nathan; Soltani, Mohammad; Salter, E; Davis, James; Henzler-Wildman, Katherine; Hong, Mei. "Structure and dynamics of the drug-bound bacterial transporter EmrE in lipid bilayers"  Nat. Commun. 12, 172-172 (2021).
PubMed: 33420032

Assembly members:

Assembly members:
S64V-EmrE, polymer, 113 residues, 11972.4008 Da.
entity_P4P, non-polymer, 339.389 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S64V-EmrE: GSHMNPYIYLGGAILAEVIG TTLMKFSEGFTRLWPSVGTI ICYCASFWLLAQTLAYIPTG IAYAIWVGVGIVLISLLSWG FFGQRLDLPAIIGMMLICAG VLIINLLSRSTPH

Data sets:
Data typeCount
13C chemical shifts610
15N chemical shifts216
1H chemical shifts214

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Chain A1
2Chain B1
3tetraphenylphosphonium2

Entities:

Entity 1, Chain A 113 residues - 11972.4008 Da.

1   GLYSERHISMETASNPROTYRILETYRLEU
2   GLYGLYALAILELEUALAGLUVALILEGLY
3   THRTHRLEUMETLYSPHESERGLUGLYPHE
4   THRARGLEUTRPPROSERVALGLYTHRILE
5   ILECYSTYRCYSALASERPHETRPLEULEU
6   ALAGLNTHRLEUALATYRILEPROTHRGLY
7   ILEALATYRALAILETRPVALGLYVALGLY
8   ILEVALLEUILESERLEULEUSERTRPGLY
9   PHEPHEGLYGLNARGLEUASPLEUPROALA
10   ILEILEGLYMETMETLEUILECYSALAGLY
11   VALLEUILEILEASNLEULEUSERARGSER
12   THRPROHIS

Entity 2, tetraphenylphosphonium - C24 H20 P - 339.389 Da.

1   P4P

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