BMRB Entry 27904
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27904
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Fis1 residues 1-125 PubMed: 29239081
Deposition date: 2019-05-13 Original release date: 2019-06-13
Authors: Egner, John; Peterson, Francis
Citation: Egner, John; Jensen, Davin; Olp, Michael; Kennedy, Nolan; Volkman, Brian; Peterson, Francis; Smith, Brian; Hill, R. "Development and Validation of 2D Difference Intensity Analysis for Chemical Library Screening by Protein- Detected NMR Spectroscopy" ChemBioChem 19, 448-458 (2018).
Assembly members:
hFis1, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30
Entity Sequences (FASTA):
hFis1: MEAVLNELVSVEDLLKFEKK
FQSEKAAGSVSKSTQFEYAW
CLVRSKYNDDIRKGIVLLEE
LLPKGSKEEQRDYVFYLAVG
NYRLKEYEKALKYVRGLLQT
EPQNNQAKELERLIDKAMKK
DGLVG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 465 |
15N chemical shifts | 118 |
1H chemical shifts | 118 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | hFis1 | 1 |
Entities:
Entity 1, hFis1 125 residues - Formula weight is not available
1 | MET | GLU | ALA | VAL | LEU | ASN | GLU | LEU | VAL | SER | ||||
2 | VAL | GLU | ASP | LEU | LEU | LYS | PHE | GLU | LYS | LYS | ||||
3 | PHE | GLN | SER | GLU | LYS | ALA | ALA | GLY | SER | VAL | ||||
4 | SER | LYS | SER | THR | GLN | PHE | GLU | TYR | ALA | TRP | ||||
5 | CYS | LEU | VAL | ARG | SER | LYS | TYR | ASN | ASP | ASP | ||||
6 | ILE | ARG | LYS | GLY | ILE | VAL | LEU | LEU | GLU | GLU | ||||
7 | LEU | LEU | PRO | LYS | GLY | SER | LYS | GLU | GLU | GLN | ||||
8 | ARG | ASP | TYR | VAL | PHE | TYR | LEU | ALA | VAL | GLY | ||||
9 | ASN | TYR | ARG | LEU | LYS | GLU | TYR | GLU | LYS | ALA | ||||
10 | LEU | LYS | TYR | VAL | ARG | GLY | LEU | LEU | GLN | THR | ||||
11 | GLU | PRO | GLN | ASN | ASN | GLN | ALA | LYS | GLU | LEU | ||||
12 | GLU | ARG | LEU | ILE | ASP | LYS | ALA | MET | LYS | LYS | ||||
13 | ASP | GLY | LEU | VAL | GLY |
Samples:
sample_1: HEPES 20 mM; sodium chloride 175 mM; DTT 2 mM; sodium azide 0.02%; hFis1, [U-98% 13C; U-98% 15N], 0.96 mM
sample_conditions_1: ionic strength: 0.175 M; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HCACO | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
GARANT, Bartels, Guntert, Billeter and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance III 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts