BMRB Entry 27913
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27913
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal Fragment of a Kinesin KIF4A Variant PubMed: 32195664
Deposition date: 2019-05-15 Original release date: 2020-03-17
Authors: Wang, Xinru; Peti, Wolfgang; Page, Rebecca
Citation: Wang, Xinru; Garvanska, Dimitriya; Nasa, Isha; Ueki, Yumi; Zhang, Gang; Kettenbach, Arminja; Peti, Wolfgang; Nilsson, Jakob; Page, Rebecca. "A dynamic charge:charge interaction modulates PP2A:B56 interactions" Elife 9, .-. (2020).
Assembly members:
KIF4A, polymer, 44 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT
Entity Sequences (FASTA):
KIF4A: GHMELKHVATEYQENKAPGK
KKKRALASNTSFFSGLEPIE
EEPE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 76 |
| 15N chemical shifts | 39 |
| 1H chemical shifts | 39 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KIF4A, A | 1 |
| 2 | KIF4A, B | 1 |
Entities:
Entity 1, KIF4A, A 44 residues - Formula weight is not available
Residues 1-3 are from a cloning artifact.
| 1 | GLY | HIS | MET | GLU | LEU | LYS | HIS | VAL | ALA | THR | ||||
| 2 | GLU | TYR | GLN | GLU | ASN | LYS | ALA | PRO | GLY | LYS | ||||
| 3 | LYS | LYS | LYS | ARG | ALA | LEU | ALA | SER | ASN | THR | ||||
| 4 | SER | PHE | PHE | SER | GLY | LEU | GLU | PRO | ILE | GLU | ||||
| 5 | GLU | GLU | PRO | GLU |
Samples:
sample_1: KIF4A, [U-99% 15N], 0.1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.5 mM; H2O 90%; D2O 10%
sample_2: KIF4A, [U-99% 13C; U-99% 15N], 3 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Neo 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts