BMRB Entry 27914

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27914

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for AIMP2 121-320 double-mutant (C205S,C291S)
Deposition date:
2019-05-16
Original release date:
2019-09-20
Authors:
Cho, Hye Young; Jeon, Young Ho; Mushtaq, Ameeq
Citation:

Citation: Lim, Semi; Cho, Hye Young; Kim, Dae Gyu; Roh, Younah; Son, Se-Young; Mushtaq, Ameeq Ul; Kim, Minkyoung; Bhattarai, Deepak; Sivaraman, Aneesh; Lee, Youngjin; Lee, Jihye; Yang, Won Suk; Kim, Hoi Kyoung; Kim, Myung Hee; Lee, Kyeong; Jeon, Young Ho; Kim, Sunghoon. "Targeting the interaction of AIMP2-DX2 with HSP70 suppresses cancer development"  Nat. Chem. Biol. 16, 31-41 (2020).
PubMed: 31792442

Assembly members:

Assembly members:
AIMP2 121-320 double-mutant (C205S,C291S), polymer, 200 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AIMP2 121-320 double-mutant (C205S,C291S): DIVINANPASPPLSLLVLHR LLCEHFRVLSTVHTHSSVKS VPENLLKCFGEQNKKQPRQD YQLGFTLIWKNVPKTQMKFS IQTMSPIEGEGNIARFLFSL FGQKHNAVNATLIDSWVDIA IFQLKEGSSKEKAAVFRSMN SALGKSPWLAGNELTVADVV LWSVLQQIGGSSVTVPANVQ RWMRSCENLAPFNTALKLLK

Data sets:
Data typeCount
13C chemical shifts462
15N chemical shifts190
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AIMP2 121-320 double-mutant (C205S,C291S)1

Entities:

Entity 1, AIMP2 121-320 double-mutant (C205S,C291S) 200 residues - Formula weight is not available

1   ASPILEVALILEASNALAASNPROALASER
2   PROPROLEUSERLEULEUVALLEUHISARG
3   LEULEUCYSGLUHISPHEARGVALLEUSER
4   THRVALHISTHRHISSERSERVALLYSSER
5   VALPROGLUASNLEULEULYSCYSPHEGLY
6   GLUGLNASNLYSLYSGLNPROARGGLNASP
7   TYRGLNLEUGLYPHETHRLEUILETRPLYS
8   ASNVALPROLYSTHRGLNMETLYSPHESER
9   ILEGLNTHRMETSERPROILEGLUGLYGLU
10   GLYASNILEALAARGPHELEUPHESERLEU
11   PHEGLYGLNLYSHISASNALAVALASNALA
12   THRLEUILEASPSERTRPVALASPILEALA
13   ILEPHEGLNLEULYSGLUGLYSERSERLYS
14   GLULYSALAALAVALPHEARGSERMETASN
15   SERALALEUGLYLYSSERPROTRPLEUALA
16   GLYASNGLULEUTHRVALALAASPVALVAL
17   LEUTRPSERVALLEUGLNGLNILEGLYGLY
18   SERSERVALTHRVALPROALAASNVALGLN
19   ARGTRPMETARGSERCYSGLUASNLEUALA
20   PROPHEASNTHRALALEULYSLEULEULYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks