BMRB Entry 27922
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27922
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Title: scAtg3(del 1-18, 86-159, 248-278) PubMed: 31399562
Deposition date: 2019-05-23 Original release date: 2019-08-06
Authors: Royappa, Grace; Zheng, Yumei; Yu, Qiu; Schulman, Brenda
Citation: Zheng, Yumei; Qiu, Yu; Grace, Christy; Liu, Xu; Klionsky, Daniel; Schulman, Brenda. "A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade" Nat. Commun. 10, 3600-3600 (2019).
Assembly members:
Atg3, polymer, 189 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae Vector: pGEX-4T-1
Entity Sequences (FASTA):
Atg3: GSKSTFLTTGQITPEEFVQA
GDYLAHMFPTWKWNEESSDI
SYRDFLPKNKQFLIIRKVPA
DKRAEQAVEAKDMAQERYYD
LYIAYSTSYRVPKMYIVGFN
SNGSPLSPEQMFEDISADYR
TKTATIEKLPFYKNSVLSVS
IHPCKHANVMKILLDKVDSL
RVDQYLIVFLKFITSVTPSI
QHDYTMEGW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 233 |
| 15N chemical shifts | 143 |
| 1H chemical shifts | 143 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Atg3 | 1 |
Entities:
Entity 1, Atg3 189 residues - Formula weight is not available
| 1 | GLY | SER | LYS | SER | THR | PHE | LEU | THR | THR | GLY | ||||
| 2 | GLN | ILE | THR | PRO | GLU | GLU | PHE | VAL | GLN | ALA | ||||
| 3 | GLY | ASP | TYR | LEU | ALA | HIS | MET | PHE | PRO | THR | ||||
| 4 | TRP | LYS | TRP | ASN | GLU | GLU | SER | SER | ASP | ILE | ||||
| 5 | SER | TYR | ARG | ASP | PHE | LEU | PRO | LYS | ASN | LYS | ||||
| 6 | GLN | PHE | LEU | ILE | ILE | ARG | LYS | VAL | PRO | ALA | ||||
| 7 | ASP | LYS | ARG | ALA | GLU | GLN | ALA | VAL | GLU | ALA | ||||
| 8 | LYS | ASP | MET | ALA | GLN | GLU | ARG | TYR | TYR | ASP | ||||
| 9 | LEU | TYR | ILE | ALA | TYR | SER | THR | SER | TYR | ARG | ||||
| 10 | VAL | PRO | LYS | MET | TYR | ILE | VAL | GLY | PHE | ASN | ||||
| 11 | SER | ASN | GLY | SER | PRO | LEU | SER | PRO | GLU | GLN | ||||
| 12 | MET | PHE | GLU | ASP | ILE | SER | ALA | ASP | TYR | ARG | ||||
| 13 | THR | LYS | THR | ALA | THR | ILE | GLU | LYS | LEU | PRO | ||||
| 14 | PHE | TYR | LYS | ASN | SER | VAL | LEU | SER | VAL | SER | ||||
| 15 | ILE | HIS | PRO | CYS | LYS | HIS | ALA | ASN | VAL | MET | ||||
| 16 | LYS | ILE | LEU | LEU | ASP | LYS | VAL | ASP | SER | LEU | ||||
| 17 | ARG | VAL | ASP | GLN | TYR | LEU | ILE | VAL | PHE | LEU | ||||
| 18 | LYS | PHE | ILE | THR | SER | VAL | THR | PRO | SER | ILE | ||||
| 19 | GLN | HIS | ASP | TYR | THR | MET | GLU | GLY | TRP |
Samples:
sample_1: Atg3, [U-100% 13C; U-100% 15N], 0.75 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - data analysis
CARA, Keller and Wuthrich - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts