BMRB Entry 27922

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27922

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Title:
scAtg3(del 1-18, 86-159, 248-278)
Deposition date:
2019-05-23
Original release date:
2019-08-06
Authors:
Royappa, Grace; Zheng, Yumei; Yu, Qiu; Schulman, Brenda
Citation:

Citation: Zheng, Yumei; Qiu, Yu; Grace, Christy; Liu, Xu; Klionsky, Daniel; Schulman, Brenda. "A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade"  Nat. Commun. 10, 3600-3600 (2019).
PubMed: 31399562

Assembly members:

Assembly members:
Atg3, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Saccharomyces cerevisiae   Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Atg3: GSKSTFLTTGQITPEEFVQA GDYLAHMFPTWKWNEESSDI SYRDFLPKNKQFLIIRKVPA DKRAEQAVEAKDMAQERYYD LYIAYSTSYRVPKMYIVGFN SNGSPLSPEQMFEDISADYR TKTATIEKLPFYKNSVLSVS IHPCKHANVMKILLDKVDSL RVDQYLIVFLKFITSVTPSI QHDYTMEGW

Data sets:
Data typeCount
13C chemical shifts233
15N chemical shifts143
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Atg31

Entities:

Entity 1, Atg3 189 residues - Formula weight is not available

1   GLYSERLYSSERTHRPHELEUTHRTHRGLY
2   GLNILETHRPROGLUGLUPHEVALGLNALA
3   GLYASPTYRLEUALAHISMETPHEPROTHR
4   TRPLYSTRPASNGLUGLUSERSERASPILE
5   SERTYRARGASPPHELEUPROLYSASNLYS
6   GLNPHELEUILEILEARGLYSVALPROALA
7   ASPLYSARGALAGLUGLNALAVALGLUALA
8   LYSASPMETALAGLNGLUARGTYRTYRASP
9   LEUTYRILEALATYRSERTHRSERTYRARG
10   VALPROLYSMETTYRILEVALGLYPHEASN
11   SERASNGLYSERPROLEUSERPROGLUGLN
12   METPHEGLUASPILESERALAASPTYRARG
13   THRLYSTHRALATHRILEGLULYSLEUPRO
14   PHETYRLYSASNSERVALLEUSERVALSER
15   ILEHISPROCYSLYSHISALAASNVALMET
16   LYSILELEULEUASPLYSVALASPSERLEU
17   ARGVALASPGLNTYRLEUILEVALPHELEU
18   LYSPHEILETHRSERVALTHRPROSERILE
19   GLNHISASPTYRTHRMETGLUGLYTRP

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