BMRB Entry 27923

Name: scAtg3FR(86-159)
Published: 2019-08-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27923

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: scAtg3FR(86-159) PubMed: 31399562

Deposition date: 2019-05-23 Original release date: 2019-08-06

Authors: Royappa, Grace; Zheng, Yumei; Yu, Qiu; Schulman, Brenda

Citation: Zheng, Yumei; Qiu, Yu; Grace, Christy; Liu, Xu; Klionsky, Daniel; Schulman, Brenda. "A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade" Nat. Commun. 10, 3600-3600 (2019).

Assembly members:
scAtg3FR(86-159), polymer, 76 residues, Formula weight is not available

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae Vector: pGEX-4T-1

Entity Sequences (FASTA):
scAtg3FR(86-159): GSVEGPDVIMKGFAEDGDED DVLEYIGSETEHVQSTPAGG TKDSSIDDIDELIQDMEIKE EDENDDTEEFNAKGGL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	205
15N chemical shifts	71
1H chemical shifts	71

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	scAtg3FR(86-159)	1

Entities:

Entity 1, scAtg3FR(86-159) 76 residues - Formula weight is not available

1

GLY

SER

VAL

GLU

GLY

PRO

ASP

VAL

ILE

MET

2

LYS

GLY

PHE

ALA

GLU

ASP

GLY

ASP

GLU

ASP

3

ASP

VAL

LEU

GLU

TYR

ILE

GLY

SER

GLU

THR

4

GLU

HIS

VAL

GLN

SER

THR

PRO

ALA

GLY

5

THR

LYS

ASP

SER

ILE

ASP

ILE

ASP

6

GLU

LEU

ILE

GLN

ASP

MET

GLU

ILE

LYS

GLU

7

GLU

ASP

GLU

ASN

ASP

THR

GLU

PHE

8

ASN

ALA

LYS

GLY

LEU

Samples:

sample_1: scAtg3FR(86-159), [U-100% 13C; U-100% 15N], 0.75 mM; MES 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(COCA)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts