BMRB Entry 27937
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27937
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Title: NMR backbone assignment of full-length human 4E-BP1 I15A F114A mutant PubMed: 33852892
Deposition date: 2019-06-06 Original release date: 2021-04-21
Authors: Boehm, Raphael; Hiller, Sebastian
Citation: Boehm, Raphael; Imseng, Stefan; Jakob, Roman; Hall, Michael; Maier, Timm; Hiller, Sebastian. "The dynamic mechanism of 4E-BP1 recognition and phosphorylation by mTORC1" Mol. Cell 81, 2403-2416 (2021).
Assembly members:
4E-BP1, polymer, 119 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
4E-BP1: GMSGGSSCSQTPSRAAPATR
RVVLGDGVQLPPGDYSTTPG
GTLFSTTPGGTRIIYDRKFL
MECRNSPVTKTPPRDLPTIP
GVTSPSSDEPPMEASQSHLR
NSPEDKRAGGEESQAEMDI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 193 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 4E-BP1 | 1 |
Entities:
Entity 1, 4E-BP1 119 residues - Formula weight is not available
Residue 1 is non-native
| 1 | GLY | MET | SER | GLY | GLY | SER | SER | CYS | SER | GLN | ||||
| 2 | THR | PRO | SER | ARG | ALA | ALA | PRO | ALA | THR | ARG | ||||
| 3 | ARG | VAL | VAL | LEU | GLY | ASP | GLY | VAL | GLN | LEU | ||||
| 4 | PRO | PRO | GLY | ASP | TYR | SER | THR | THR | PRO | GLY | ||||
| 5 | GLY | THR | LEU | PHE | SER | THR | THR | PRO | GLY | GLY | ||||
| 6 | THR | ARG | ILE | ILE | TYR | ASP | ARG | LYS | PHE | LEU | ||||
| 7 | MET | GLU | CYS | ARG | ASN | SER | PRO | VAL | THR | LYS | ||||
| 8 | THR | PRO | PRO | ARG | ASP | LEU | PRO | THR | ILE | PRO | ||||
| 9 | GLY | VAL | THR | SER | PRO | SER | SER | ASP | GLU | PRO | ||||
| 10 | PRO | MET | GLU | ALA | SER | GLN | SER | HIS | LEU | ARG | ||||
| 11 | ASN | SER | PRO | GLU | ASP | LYS | ARG | ALA | GLY | GLY | ||||
| 12 | GLU | GLU | SER | GLN | ALA | GLU | MET | ASP | ILE |
Samples:
sample_1: 4E-BP1, [U-99% 13C; U-99% 15N], 250 uM; sodium phosphate 30 mM; sodium chloride 160 mM; EDTA 1 mM; DTT 10 mM
sample_conditions_1: ionic strength: 0.19 M; pH: 6; pressure: 1 atm; temperature: 293.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts