BMRB Entry 27955

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27955

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
ILVM methyl assignments for Pyrococcus furiosus Rad50 NBD (wild type) with unlabeled Mre11
Deposition date:
2019-06-24
Original release date:
2019-07-23
Authors:
Boswell, Zachary; Canny, Marella; Sang, Julie; Latham, Michael
Citation:

Citation: Boswell, Zachary; Canny, Marella; Buschmann, Tanner; Sang, Julie; Latham, Michael. "Adjacent mutations in the archaeal Rad50 ABC ATPase D-loop disrupt allosteric regulation of ATP hydrolysis through different mechanisms"  Nucleic Acids Res. 48, 2457-2472 (2020).
PubMed: 31889185

Assembly members:

Assembly members:
Pf_Rad50_NBD_(wild_type)_A, polymer, 199 residues, Formula weight is not available
Pf_Rad50_NBD_(wild_type)_B, polymer, 174 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pf_Rad50_NBD_(wild_type)_A: AMGSMKLERVTVKNFRSHSD TVVEFKEGINLIIGQNGSGK SSLLDAILVGLYWPLRIKDI KKDEFTKVGARDTYIDLIFE KDGTKYRITRRFLKGYSSGE IHAMKRLVGNEWKHVTEPSS KAISAFMEKLIPYNIFLNAI YIRQGQIDAILESDEAREKV VREVLNLDKFETAYKKLSEL KKTINNRIKEYRDILARTE
Pf_Rad50_NBD_(wild_type)_B: RERVKKEIKDLEKAKDFTEE LIEKVKKYKALAREAALSKI GELASEIFAEFTEGKYSEVV VRAEENKVRLFVVWEGKERP LTFLSGGERIALGLAFRLAM SLYLAGEISLLILDEPTPYL DEERRRKLITIMERYLKKIP QVILVSHDEELKDAADHVIR ISLENGSSKVEVVS

Data typeCount
13C chemical shifts138
1H chemical shifts411

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rad50NBD, subunit A1
2Rad50NBD, subunit B2

Entities:

Entity 1, Rad50NBD, subunit A 199 residues - Formula weight is not available

1   ALAMETGLYSERMETLYSLEUGLUARGVAL
2   THRVALLYSASNPHEARGSERHISSERASP
3   THRVALVALGLUPHELYSGLUGLYILEASN
4   LEUILEILEGLYGLNASNGLYSERGLYLYS
5   SERSERLEULEUASPALAILELEUVALGLY
6   LEUTYRTRPPROLEUARGILELYSASPILE
7   LYSLYSASPGLUPHETHRLYSVALGLYALA
8   ARGASPTHRTYRILEASPLEUILEPHEGLU
9   LYSASPGLYTHRLYSTYRARGILETHRARG
10   ARGPHELEULYSGLYTYRSERSERGLYGLU
11   ILEHISALAMETLYSARGLEUVALGLYASN
12   GLUTRPLYSHISVALTHRGLUPROSERSER
13   LYSALAILESERALAPHEMETGLULYSLEU
14   ILEPROTYRASNILEPHELEUASNALAILE
15   TYRILEARGGLNGLYGLNILEASPALAILE
16   LEUGLUSERASPGLUALAARGGLULYSVAL
17   VALARGGLUVALLEUASNLEUASPLYSPHE
18   GLUTHRALATYRLYSLYSLEUSERGLULEU
19   LYSLYSTHRILEASNASNARGILELYSGLU
20   TYRARGASPILELEUALAARGTHRGLU

Entity 2, Rad50NBD, subunit B 174 residues - Formula weight is not available

1   ARGGLUARGVALLYSLYSGLUILELYSASP
2   LEUGLULYSALALYSASPPHETHRGLUGLU
3   LEUILEGLULYSVALLYSLYSTYRLYSALA
4   LEUALAARGGLUALAALALEUSERLYSILE
5   GLYGLULEUALASERGLUILEPHEALAGLU
6   PHETHRGLUGLYLYSTYRSERGLUVALVAL
7   VALARGALAGLUGLUASNLYSVALARGLEU
8   PHEVALVALTRPGLUGLYLYSGLUARGPRO
9   LEUTHRPHELEUSERGLYGLYGLUARGILE
10   ALALEUGLYLEUALAPHEARGLEUALAMET
11   SERLEUTYRLEUALAGLYGLUILESERLEU
12   LEUILELEUASPGLUPROTHRPROTYRLEU
13   ASPGLUGLUARGARGARGLYSLEUILETHR
14   ILEMETGLUARGTYRLEULYSLYSILEPRO
15   GLNVALILELEUVALSERHISASPGLUGLU
16   LEULYSASPALAALAASPHISVALILEARG
17   ILESERLEUGLUASNGLYSERSERLYSVAL
18   GLUVALVALSER