BMRB Entry 27973

Name: Solid-state NMR Chemical Shift Assignments for p150Glued(1-191) of Dynactin
Published: 2019-08-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27973

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR Chemical Shift Assignments for p150Glued(1-191) of Dynactin

Deposition date:

2019-07-12

Original release date:

2019-08-15

Authors:

Guo, Changmiao; Polenova, Tatyana; Williams, John

Citation:

Citation: Guo, Changmiao; Williams, John; Polenova, Tatyana. "Conformational Flexibility of p150Glued(1-191) Subunit of Dynactin Assembled with Microtubules" Biophys. J. 117, 938-949 (2019).
PubMed: 31445682

Assembly members:

Assembly members:
p150Glued(1-191), polymer, 191 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p150Glued(1-191): MAQSKRHMYNRTPSGSRMST EASARPLRVGSRVEVIGKGH RGTVAYVGATLFATGKWVGV ILDEAKGKNDGTVQGRKYFT CDEGHGIFVRQSQIQVFEDG ADTTSPETPDSSASKILKRE GADAAAKTSKLRGLKPKKAP TARKTTTRRPKPTRPASTGV AGPSSSLGPSGSASAGELSS SEPSTPAQTPL

Data sets:

assigned_chemical_shifts
- 13_and_15N_chemical_shifts_of_p150Glued(1-191)_assembled_on_microtubules

Data type	Count
13C chemical shifts	296

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p150Glued(1-191) Subunit of Dynactin	1

Entities:

Entity 1, p150Glued(1-191) Subunit of Dynactin 191 residues - Formula weight is not available

1

MET

ALA

GLN

SER

LYS

ARG

HIS

MET

TYR

ASN

2

ARG

THR

PRO

SER

GLY

SER

ARG

MET

SER

THR

3

GLU

ALA

SER

ALA

ARG

PRO

LEU

ARG

VAL

GLY

4

SER

ARG

VAL

GLU

VAL

ILE

GLY

LYS

GLY

HIS

5

ARG

GLY

THR

VAL

ALA

TYR

VAL

GLY

ALA

THR

6

LEU

PHE

ALA

THR

GLY

LYS

TRP

VAL

GLY

VAL

7

ILE

LEU

ASP

GLU

ALA

LYS

GLY

LYS

ASN

ASP

8

GLY

THR

VAL

GLN

GLY

ARG

LYS

TYR

PHE

THR

9

CYS

ASP

GLU

GLY

HIS

GLY

ILE

PHE

VAL

ARG

10

GLN

SER

GLN

ILE

GLN

VAL

PHE

GLU

ASP

GLY

11

ALA

ASP

THR

SER

PRO

GLU

THR

PRO

ASP

12

SER

ALA

SER

LYS

ILE

LEU

LYS

ARG

GLU

13

GLY

ALA

ASP

ALA

LYS

THR

SER

LYS

14

LEU

ARG

GLY

LEU

LYS

PRO

LYS

ALA

PRO

15

THR

ALA

ARG

LYS

THR

ARG

PRO

16

LYS

PRO

THR

ARG

PRO

ALA

SER

THR

GLY

VAL

17

ALA

GLY

PRO

SER

LEU

GLY

PRO

SER

18

GLY

SER

ALA

SER

ALA

GLY

GLU

LEU

SER

19

SER

GLU

PRO

SER

THR

PRO

ALA

GLN

THR

PRO

20

LEU

Samples:

sample_complexes: p150Glued(1-191), [U-100% 13C; U-100% 15N], 450 uM; microtubules 540 uM; PIPES 80 mM; MgCl2 1 mM; EGTA 1 mM

sample_microtubules: microtubules 540 uM; PIPES 80 mM; MgCl2 1 mM; EGTA 1 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

sample_conditions_2: pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C CORD	sample_complexes	isotropic	sample_conditions_1
2D NCA	sample_complexes	isotropic	sample_conditions_1
2D NCO	sample_complexes	isotropic	sample_conditions_1
1H-31P CPMAS	sample_microtubules	isotropic	sample_conditions_2
31P direct polarization MAS NMR	sample_microtubules	isotropic	sample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR, Varian - collection

NMR spectrometers:

Varian Uniform NMR System 600 MHz