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BMRB Entry 27997

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27997

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Title: Chemical shift assignments for RCAN1 residues 128-164 in complex with calcineurin catalytic subunit A residues 27-348   PubMed: 32936779

Deposition date: 2019-08-12 Original release date: 2020-07-09

Authors: Peti, Wolfgang; Li, Yang; Page, Rebecca; Sheftic, Sarah

Citation: Li, Yang; Sheftic, Sarah; Grigoriu, Simina; Schwieters, Charles; Page, Rebecca; Peti, Wolfgang. "The structure of the RCAN1:CN complex explains the inhibition of and substrate recruitment by calcineurin"  Sci. Adv. 6, 3681-3681 (2020).

Assembly members:
RCAN1_residues_128-164, polymer, 40 residues, Formula weight is not available
Calcineurin_catalytic_subunit_A_residues_27-348, polymer, 327 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTHMT

Entity Sequences (FASTA):
RCAN1_residues_128-164: GHMNYDLLYAISKLGPGEKY ELHAATDTTPSVVITVCESD
Calcineurin_catalytic_subunit_A_residues_27-348: GHMHRLTAKEVFDNDGKPRV DILKAHLMKEGRLEESVALR IITEGASILRQEKNLLDIDA PVTVCGDIHGQFFDLMKLFE VGGSPANTRYLFLGDYVDRG YFSIECVLYLWALKILYPKT LFLLRGNHECRHLTEYFTFK QECKIKYSERVYDACMDAFD CLPLAALMNQQFLCVHGGLS PEINTLDDIRKLDRFKEPPA YGPMCDILWSDPLEDFGNEK TQEHFTHNTVRGCSYFYSYP AVCEFLQHNNLLSILRAHEA QDAGYRMYRKSQTTGFPSLI TIFSAPNYLDVYNNKAAVLK YENNVMNIRQFNCSPHPYWL PNFMDDD

Data sets:
Data typeCount
13C chemical shifts83
15N chemical shifts35
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RCAN11
2Calcineurin subunit A2

Entities:

Entity 1, RCAN1 40 residues - Formula weight is not available

GHM are cloning artifact; RCAN1 sequence starts with NYDL

1   GLYHISMETASNTYRASPLEULEUTYRALA
2   ILESERLYSLEUGLYPROGLYGLULYSTYR
3   GLULEUHISALAALATHRASPTHRTHRPRO
4   SERVALVALILETHRVALCYSGLUSERASP

Entity 2, Calcineurin subunit A 327 residues - Formula weight is not available

GHM are cloning artifact; calcineurin sequence starts with HRLT; the C-terminal DD are introduced to increase solubility.

1   GLYHISMETHISARGLEUTHRALALYSGLU
2   VALPHEASPASNASPGLYLYSPROARGVAL
3   ASPILELEULYSALAHISLEUMETLYSGLU
4   GLYARGLEUGLUGLUSERVALALALEUARG
5   ILEILETHRGLUGLYALASERILELEUARG
6   GLNGLULYSASNLEULEUASPILEASPALA
7   PROVALTHRVALCYSGLYASPILEHISGLY
8   GLNPHEPHEASPLEUMETLYSLEUPHEGLU
9   VALGLYGLYSERPROALAASNTHRARGTYR
10   LEUPHELEUGLYASPTYRVALASPARGGLY
11   TYRPHESERILEGLUCYSVALLEUTYRLEU
12   TRPALALEULYSILELEUTYRPROLYSTHR
13   LEUPHELEULEUARGGLYASNHISGLUCYS
14   ARGHISLEUTHRGLUTYRPHETHRPHELYS
15   GLNGLUCYSLYSILELYSTYRSERGLUARG
16   VALTYRASPALACYSMETASPALAPHEASP
17   CYSLEUPROLEUALAALALEUMETASNGLN
18   GLNPHELEUCYSVALHISGLYGLYLEUSER
19   PROGLUILEASNTHRLEUASPASPILEARG
20   LYSLEUASPARGPHELYSGLUPROPROALA
21   TYRGLYPROMETCYSASPILELEUTRPSER
22   ASPPROLEUGLUASPPHEGLYASNGLULYS
23   THRGLNGLUHISPHETHRHISASNTHRVAL
24   ARGGLYCYSSERTYRPHETYRSERTYRPRO
25   ALAVALCYSGLUPHELEUGLNHISASNASN
26   LEULEUSERILELEUARGALAHISGLUALA
27   GLNASPALAGLYTYRARGMETTYRARGLYS
28   SERGLNTHRTHRGLYPHEPROSERLEUILE
29   THRILEPHESERALAPROASNTYRLEUASP
30   VALTYRASNASNLYSALAALAVALLEULYS
31   TYRGLUASNASNVALMETASNILEARGGLN
32   PHEASNCYSSERPROHISPROTYRTRPLEU
33   PROASNPHEMETASPASPASP

Samples:

sample_1: RCAN1 residues 128-164, [U-13C; U-15N; U-2H], 0.35 mM; calcineurin catalytic subunit A, residues 27-348 0.35 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.5 mM

sample_2: RCAN1 residues 128-164, [ILV-13CH3; U-99% 2H; U-99% 15N], 0.4 mM; calcineurin catalytic subunit A, residues 27-348 0.4 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

cara, Keller and Wuthrich - chemical shift assignment

ccpnmr, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 850 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP P53805-2 Q08209 P53805-2
AlphaFold Q9UME4 Q8TAW9 Q9UME4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts