BMRB Entry 27999
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27999
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Title: Structural basis for client recognition and activity of Hsp40 chaperones PubMed: 31604242
Deposition date: 2019-08-16 Original release date: 2019-11-07
Authors: Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos
Citation: Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos. "Structural basis for client recognition and activity of Hsp40 chaperones" Science 365, 1313-1319 (2019).
Assembly members:
Sis1_SBD, polymer, 158 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet16b
Entity Sequences (FASTA):
Sis1_SBD: EEETVQVNLPVSLEDLFVGK
KKSFKIGRKGPHGASEKTQI
DIQLKPGWKAGTKITYKNQG
DYNPQTGRRKTLQFVIQEKS
HPNFKRDGDDLIYTLPLSFK
ESLLGFSKTIQTIDGRTLPL
SRVQPVQPSQTSTYPGQGMP
TPKNPSQRGNLIVKYKVD
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 138 |
| 1H chemical shifts | 138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sis1_SBD | 1 |
Entities:
Entity 1, Sis1_SBD 158 residues - Formula weight is not available
| 1 | GLU | GLU | GLU | THR | VAL | GLN | VAL | ASN | LEU | PRO | ||||
| 2 | VAL | SER | LEU | GLU | ASP | LEU | PHE | VAL | GLY | LYS | ||||
| 3 | LYS | LYS | SER | PHE | LYS | ILE | GLY | ARG | LYS | GLY | ||||
| 4 | PRO | HIS | GLY | ALA | SER | GLU | LYS | THR | GLN | ILE | ||||
| 5 | ASP | ILE | GLN | LEU | LYS | PRO | GLY | TRP | LYS | ALA | ||||
| 6 | GLY | THR | LYS | ILE | THR | TYR | LYS | ASN | GLN | GLY | ||||
| 7 | ASP | TYR | ASN | PRO | GLN | THR | GLY | ARG | ARG | LYS | ||||
| 8 | THR | LEU | GLN | PHE | VAL | ILE | GLN | GLU | LYS | SER | ||||
| 9 | HIS | PRO | ASN | PHE | LYS | ARG | ASP | GLY | ASP | ASP | ||||
| 10 | LEU | ILE | TYR | THR | LEU | PRO | LEU | SER | PHE | LYS | ||||
| 11 | GLU | SER | LEU | LEU | GLY | PHE | SER | LYS | THR | ILE | ||||
| 12 | GLN | THR | ILE | ASP | GLY | ARG | THR | LEU | PRO | LEU | ||||
| 13 | SER | ARG | VAL | GLN | PRO | VAL | GLN | PRO | SER | GLN | ||||
| 14 | THR | SER | THR | TYR | PRO | GLY | GLN | GLY | MET | PRO | ||||
| 15 | THR | PRO | LYS | ASN | PRO | SER | GLN | ARG | GLY | ASN | ||||
| 16 | LEU | ILE | VAL | LYS | TYR | LYS | VAL | ASP |
Samples:
sample_1: Sis1_SBD, [U-100% 13C; U-100% 15N], 700 uM; potassium chloride 75 mM; potassium phosphate 20 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - data analysis
PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts