BMRB Entry 28011

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR28011

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Mdm2 (residues 284 to 434)
Deposition date:
2019-09-08
Original release date:
2019-10-15
Authors:
Theillet, Francois-Xavier
Citation:

Citation: Alik, Ania; Bouguechtouli, Chafiaa; Julien, Manon; Bermel, Wolfgang; Ghouil, Rania; Zinn-Justin, Sophie; Theillet, Francois-Xavier. "Sensitivity-Enhanced 13 C-NMR Spectroscopy for Monitoring Multisite Phosphorylation at Physiological Temperature and pH"  Angew. Chem. Int. Ed. Engl. 59, 10411-10415 (2020).
PubMed: 32181947

Assembly members:

Assembly members:
Mdm2, polymer, 151 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mdm2: GESDTDSFEEDPEISLADYW KCTSCNEMNPPLPSHCNRCW ALRENWLPEDKGKDKGEISE KAKLENSTQAEEGFDVPDAK KTIVNDSRESAVEENDDKIT QASQSQESEDYSQPSTSSSI IYSSQEDVKEFEREETQDKE ESVESSLPLNA

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts139
1H chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Mdm21
2ligand2

Entities:

Entity 1, Mdm2 151 residues - Formula weight is not available

1   GLYGLUSERASPTHRASPSERPHEGLUGLU
2   ASPPROGLUILESERLEUALAASPTYRTRP
3   LYSCYSTHRSERCYSASNGLUMETASNPRO
4   PROLEUPROSERHISCYSASNARGCYSTRP
5   ALALEUARGGLUASNTRPLEUPROGLUASP
6   LYSGLYLYSASPLYSGLYGLUILESERGLU
7   LYSALALYSLEUGLUASNSERTHRGLNALA
8   GLUGLUGLYPHEASPVALPROASPALALYS
9   LYSTHRILEVALASNASPSERARGGLUSER
10   ALAVALGLUGLUASNASPASPLYSILETHR
11   GLNALASERGLNSERGLNGLUSERGLUASP
12   TYRSERGLNPROSERTHRSERSERSERILE
13   ILETYRSERSERGLNGLUASPVALLYSGLU
14   PHEGLUARGGLUGLUTHRGLNASPLYSGLU
15   GLUSERVALGLUSERSERLEUPROLEUASN
16   ALA

Entity 2, ligand - Zn - 65.409 Da.

1   ZN

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks