BMRB Entry 28014
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28014
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Title: Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments of RimP from E. coli PubMed: 32303998
Deposition date: 2019-09-11 Original release date: 2020-12-14
Authors: Schedlbauer, Andreas; Kaminishi, Tatsuya; Iturrioz, Idoia; Ochoa Lizarralde, Borja; Gil-Carton, David; de Astigarraga, Elisa; Diercks, Tammo; Fucini, Paola; Connell, Sean
Citation: Schedlbauer, Andreas; Ochoa-Lizarralde, Borja; Iturrioz, Idoia; Capuni, Retina; Diercks, Tammo; de Astigarraga, Elisa; Fucini, Paola; Connell, Sean. "Backbone and sidechain NMR assignments for the ribosome maturation factor RimP from Escherichia coli" Biomol. NMR Assign. 14, 189-193 (2020).
Assembly members:
RimP, polymer, 151 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p7XNH3
Entity Sequences (FASTA):
RimP: MSTLEQKLTEMITAPVEALG
FELVGIEFIRGRTSTLRIYI
DSEDGINVDDCADVSHQVSA
VLDVEDPITVAYNLEVSSPG
LDRPLFTAEHYARFVGEEVT
LVLRMAVQNRRKWQGVIKAV
DGEMITVTVEGKDEVFALSN
IQKANLVPHFA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 619 |
| 15N chemical shifts | 145 |
| 1H chemical shifts | 991 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RimP | 1 |
Entities:
Entity 1, RimP 151 residues - Formula weight is not available
| 1 | MET | SER | THR | LEU | GLU | GLN | LYS | LEU | THR | GLU | ||||
| 2 | MET | ILE | THR | ALA | PRO | VAL | GLU | ALA | LEU | GLY | ||||
| 3 | PHE | GLU | LEU | VAL | GLY | ILE | GLU | PHE | ILE | ARG | ||||
| 4 | GLY | ARG | THR | SER | THR | LEU | ARG | ILE | TYR | ILE | ||||
| 5 | ASP | SER | GLU | ASP | GLY | ILE | ASN | VAL | ASP | ASP | ||||
| 6 | CYS | ALA | ASP | VAL | SER | HIS | GLN | VAL | SER | ALA | ||||
| 7 | VAL | LEU | ASP | VAL | GLU | ASP | PRO | ILE | THR | VAL | ||||
| 8 | ALA | TYR | ASN | LEU | GLU | VAL | SER | SER | PRO | GLY | ||||
| 9 | LEU | ASP | ARG | PRO | LEU | PHE | THR | ALA | GLU | HIS | ||||
| 10 | TYR | ALA | ARG | PHE | VAL | GLY | GLU | GLU | VAL | THR | ||||
| 11 | LEU | VAL | LEU | ARG | MET | ALA | VAL | GLN | ASN | ARG | ||||
| 12 | ARG | LYS | TRP | GLN | GLY | VAL | ILE | LYS | ALA | VAL | ||||
| 13 | ASP | GLY | GLU | MET | ILE | THR | VAL | THR | VAL | GLU | ||||
| 14 | GLY | LYS | ASP | GLU | VAL | PHE | ALA | LEU | SER | ASN | ||||
| 15 | ILE | GLN | LYS | ALA | ASN | LEU | VAL | PRO | HIS | PHE | ||||
| 16 | ALA |
Samples:
sample_1: RimP, [U-98% 13C; U-98% 15N], 600 uM; HEPES 10 mM; MgCl2 6 mM; NH4Cl 30 mM; TCEP 75 uM
sample_conditions_1: pH: 7.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CNH NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNH NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCH NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCAHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CAHA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
ccpnmr v2.4, CCPN - chemical shift assignment
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts