BMRB Entry 28022

Title:
1H and 15N assignment of 15N-labeled oncogenic mutant human KRas4B-G12D(1-169) bound to GTP at physiological pH
Deposition date:
2019-09-27
Original release date:
2020-09-01
Authors:
Palfy, Gyula; Vida, Istvan; Perczel, Andras
Citation:

Citation: Menyhard, Dora; Palfy, Gyula; Orgovan, Zoltan; Vida, Istvan; Keseru, Gyorgy; Perczel, Andras. "Structural impact of GTP binding on downstream KRAS signaling"  Chem. Sci. 11, 9272-9289 (2020).

Assembly members:

Assembly members:
K-Ras-G12D, polymer, 171 residues, Formula weight is not available
GUANOSINE-5'-TRIPHOSPHATE, non-polymer, 523.180 Da.
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Data sets:
Data typeCount
15N chemical shifts129
1H chemical shifts264

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1K-Ras-G12D1
2GTP2
3Mg2+3

Entities:

Entity 1, K-Ras-G12D 171 residues - Formula weight is not available

1   GLYHISMETTHRGLUTYRLYSLEUVALVAL
2   VALGLYALAASPGLYVALGLYLYSSERALA
3   LEUTHRILEGLNLEUILEGLNASNHISPHE
4   VALASPGLUTYRASPPROTHRILEGLUASP
5   SERTYRARGLYSGLNVALVALILEASPGLY
6   GLUTHRCYSLEULEUASPILELEUASPTHR
7   ALAGLYGLNGLUGLUTYRSERALAMETARG
8   ASPGLNTYRMETARGTHRGLYGLUGLYPHE
9   LEUCYSVALPHEALAILEASNASNTHRLYS
10   SERPHEGLUASPILEHISHISTYRARGGLU
11   GLNILELYSARGVALLYSASPSERGLUASP
12   VALPROMETVALLEUVALGLYASNLYSCYS
13   ASPLEUPROSERARGTHRVALASPTHRLYS
14   GLNALAGLNASPLEUALAARGSERTYRGLY
15   ILEPROPHEILEGLUTHRSERALALYSTHR
16   ARGGLNGLYVALASPASPALAPHETYRTHR
17   LEUVALARGGLUILEARGLYSHISLYSGLU
18   LYS

Entity 2, GTP - C10 H16 N5 O14 P3 - 523.180 Da.

1   GTP

Entity 3, Mg2+ - Mg - 24.305 Da.

1   MG

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks