BMRB Entry 28032
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28032
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Chemical shifts of mouse BTNL2 IgV1 domain PubMed: 32976909
Deposition date: 2019-10-19 Original release date: 2021-07-16
Authors: Basak, Aditya; De, Soumya
Citation: Basak, Aditya; Maiti, Snigdha; Hansda, Anita; Mahata, Dhrubajyoti; Duraivelan, Kheerthana; Kundapura, Shankar; Lee, Woonghee; Mukherjee, Gayatri; De, Soumya; Samanta, Dibyendu. "Structural Insights into N-terminal IgV Domain of BTNL2, a T Cell Inhibitory Molecule, Suggests a Non-canonical Binding Interface for Its Putative Receptors" J. Mol. Biol. 432, 5938-5950 (2020).
Assembly members:
BTNL2, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 3a
Entity Sequences (FASTA):
BTNL2: MDFRVVGPNLPILAKVGEDA
LLTCQLLPKRTTAHMEVRWY
RSDPDMPVIMYRDGAEVTGL
PMEGYGGRAEWMEDSTEEGS
VALKIRQVQPSDDGQYWCRF
QEGDYWRETSVLLQVAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 452 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 663 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BTNL2 Igv1 monomer | 1 |
Entities:
Entity 1, BTNL2 Igv1 monomer 117 residues - Formula weight is not available
| 1 | MET | ASP | PHE | ARG | VAL | VAL | GLY | PRO | ASN | LEU | ||||
| 2 | PRO | ILE | LEU | ALA | LYS | VAL | GLY | GLU | ASP | ALA | ||||
| 3 | LEU | LEU | THR | CYS | GLN | LEU | LEU | PRO | LYS | ARG | ||||
| 4 | THR | THR | ALA | HIS | MET | GLU | VAL | ARG | TRP | TYR | ||||
| 5 | ARG | SER | ASP | PRO | ASP | MET | PRO | VAL | ILE | MET | ||||
| 6 | TYR | ARG | ASP | GLY | ALA | GLU | VAL | THR | GLY | LEU | ||||
| 7 | PRO | MET | GLU | GLY | TYR | GLY | GLY | ARG | ALA | GLU | ||||
| 8 | TRP | MET | GLU | ASP | SER | THR | GLU | GLU | GLY | SER | ||||
| 9 | VAL | ALA | LEU | LYS | ILE | ARG | GLN | VAL | GLN | PRO | ||||
| 10 | SER | ASP | ASP | GLY | GLN | TYR | TRP | CYS | ARG | PHE | ||||
| 11 | GLN | GLU | GLY | ASP | TYR | TRP | ARG | GLU | THR | SER | ||||
| 12 | VAL | LEU | LEU | GLN | VAL | ALA | ALA |
Samples:
sample_1: BTNL2, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 90 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts