BMRB Entry 28036

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28036

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Title:
Shaker-VSD(S2bot_mutant)
Deposition date:
2019-11-04
Original release date:
2020-01-10
Authors:
Chen, Hongbo; Chanda, Baron; Henzler-Wildman, Katherine
Citation:

Citation: Chen, Hongbo; Deng, Jiahua; Cui, Qiang; Chanda, Baron; Henzler-Wildman, Katherine. "Mapping temperature-dependent conformational change in the voltage-sensing domain of an engineered heat-activated K + channel"  Proc. Natl. Acad. Sci. U. S. A. 118, e2017280118-e2017280118 (2021).
PubMed: 33782120

Assembly members:

Assembly members:
Shaker-VSD(S2bot_mutant), polymer, 185 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Shaker-VSD(S2bot_mutant): GGGGFEYPESSQAARVVAII SVFVILLSIVIFCLETLPEF KHYKVFNTTTNGTKIEEDEV PDITDPFFLIETLCIIWFTF ELTVRFLACPNKLNFCRDVM NVIDIIAIIPYFITLATVVA EEEDTLNLPKAPVSPQDKSS NQAMSLAILRVIRLVRSSRI FKLSRHSKGLQILGRTLKAS MRELG

Data sets:
Data typeCount
13C chemical shifts510
15N chemical shifts155
1H chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Shaker-VSD(S2bot_mutant)1

Entities:

Entity 1, Shaker-VSD(S2bot_mutant) 185 residues - Formula weight is not available

1   GLYGLYGLYGLYPHEGLUTYRPROGLUSER
2   SERGLNALAALAARGVALVALALAILEILE
3   SERVALPHEVALILELEULEUSERILEVAL
4   ILEPHECYSLEUGLUTHRLEUPROGLUPHE
5   LYSHISTYRLYSVALPHEASNTHRTHRTHR
6   ASNGLYTHRLYSILEGLUGLUASPGLUVAL
7   PROASPILETHRASPPROPHEPHELEUILE
8   GLUTHRLEUCYSILEILETRPPHETHRPHE
9   GLULEUTHRVALARGPHELEUALACYSPRO
10   ASNLYSLEUASNPHECYSARGASPVALMET
11   ASNVALILEASPILEILEALAILEILEPRO
12   TYRPHEILETHRLEUALATHRVALVALALA
13   GLUGLUGLUASPTHRLEUASNLEUPROLYS
14   ALAPROVALSERPROGLNASPLYSSERSER
15   ASNGLNALAMETSERLEUALAILELEUARG
16   VALILEARGLEUVALARGSERSERARGILE
17   PHELYSLEUSERARGHISSERLYSGLYLEU
18   GLNILELEUGLYARGTHRLEULYSALASER
19   METARGGLULEUGLY

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